Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32837 | 3' | -55.3 | NC_007346.1 | + | 60397 | 0.68 | 0.973684 |
Target: 5'- aGUAUGCUggUGUAcCCGGAGCagauaucuGCGGGAg -3' miRNA: 3'- gCAUACGG--ACGU-GGCUUCGg-------UGUCCU- -5' |
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32837 | 3' | -55.3 | NC_007346.1 | + | 195589 | 0.68 | 0.962111 |
Target: 5'- cCGUaAUGCCUGCACCaGAuGGCgCAacuGGGAc -3' miRNA: 3'- -GCA-UACGGACGUGG-CU-UCG-GUg--UCCU- -5' |
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32837 | 3' | -55.3 | NC_007346.1 | + | 254023 | 0.69 | 0.955166 |
Target: 5'- uGUcgGaUgaGCACCgGAGGUCGCAGGAg -3' miRNA: 3'- gCAuaC-GgaCGUGG-CUUCGGUGUCCU- -5' |
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32837 | 3' | -55.3 | NC_007346.1 | + | 255134 | 0.69 | 0.955166 |
Target: 5'- uGUcgGaUgaGCACCgGAGGUCGCAGGAg -3' miRNA: 3'- gCAuaC-GgaCGUGG-CUUCGGUGUCCU- -5' |
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32837 | 3' | -55.3 | NC_007346.1 | + | 322555 | 1.09 | 0.008149 |
Target: 5'- aCGUAUGCCUGCACCGAAGCCACAGGAu -3' miRNA: 3'- -GCAUACGGACGUGGCUUCGGUGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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