Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32838 | 3' | -46.6 | NC_007346.1 | + | 361796 | 0.66 | 1 |
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Target: 5'- uGAUUUucgcGCGAGcaaacucguuGCUGCUGCaCAACc -3' miRNA: 3'- -CUAAAuau-UGCUCu---------CGACGACG-GUUG- -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 156091 | 0.66 | 1 |
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Target: 5'- ------cAGCGGGuGCUGCUGCa--- -3' miRNA: 3'- cuaaauaUUGCUCuCGACGACGguug -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 253164 | 0.66 | 1 |
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Target: 5'- ----gAUGGgGAGAGgaGCgucuugGCCGACu -3' miRNA: 3'- cuaaaUAUUgCUCUCgaCGa-----CGGUUG- -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 254100 | 0.67 | 0.999999 |
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Target: 5'- -cUUUAUggUGA-AGCUGCaUGCCGAg -3' miRNA: 3'- cuAAAUAuuGCUcUCGACG-ACGGUUg -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 255211 | 0.67 | 0.999999 |
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Target: 5'- -cUUUAUggUGA-AGCUGCaUGCCGAg -3' miRNA: 3'- cuAAAUAuuGCUcUCGACG-ACGGUUg -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 172608 | 0.69 | 0.999972 |
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Target: 5'- uGAgggUcgGGCGccuGCUGCUGCCAAUa -3' miRNA: 3'- -CUaa-AuaUUGCucuCGACGACGGUUG- -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 86408 | 0.69 | 0.999934 |
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Target: 5'- uGGUgcugAACGAuGAGCUgGgUGCCAGCg -3' miRNA: 3'- -CUAaauaUUGCU-CUCGA-CgACGGUUG- -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 70915 | 0.7 | 0.999814 |
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Target: 5'- aGGUUUA--GCGAaAGCUGCUGCUGGa -3' miRNA: 3'- -CUAAAUauUGCUcUCGACGACGGUUg -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 262318 | 0.75 | 0.98845 |
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Target: 5'- ----aGUAAUGAGAGCauuUGCUGCgCGGCg -3' miRNA: 3'- cuaaaUAUUGCUCUCG---ACGACG-GUUG- -5' |
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| 32838 | 3' | -46.6 | NC_007346.1 | + | 333771 | 1.11 | 0.037663 |
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Target: 5'- aGAUUUAUAACGAGAGCUGCUGCCAACu -3' miRNA: 3'- -CUAAAUAUUGCUCUCGACGACGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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