miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32839 3' -49 NC_007346.1 + 309576 0.66 0.999994
Target:  5'- cACUG--GCUcgUCaCGGAGGcAUCGUCg -3'
miRNA:   3'- -UGACuaUGAuaGG-GUCUCCuUAGCAG- -5'
32839 3' -49 NC_007346.1 + 223940 0.66 0.999983
Target:  5'- uACUGGagGCggggAUUCCGGGGGGAUgGUa -3'
miRNA:   3'- -UGACUa-UGa---UAGGGUCUCCUUAgCAg -5'
32839 3' -49 NC_007346.1 + 177999 0.67 0.99997
Target:  5'- cACUGGUGgUAUCCUAGGu--AUUGUCa -3'
miRNA:   3'- -UGACUAUgAUAGGGUCUccuUAGCAG- -5'
32839 3' -49 NC_007346.1 + 171885 0.68 0.999811
Target:  5'- aGCUGAUACUucuuuUCCUAacaauGGAAUgCGUCg -3'
miRNA:   3'- -UGACUAUGAu----AGGGUcu---CCUUA-GCAG- -5'
32839 3' -49 NC_007346.1 + 7993 0.7 0.99897
Target:  5'- --aGAUugUGaCCCGGcgucGGAAUCGUCa -3'
miRNA:   3'- ugaCUAugAUaGGGUCu---CCUUAGCAG- -5'
32839 3' -49 NC_007346.1 + 188147 0.73 0.986483
Target:  5'- cCUGAUGCUAgUUCUGGGGGAAgugaaguuUCGUCa -3'
miRNA:   3'- uGACUAUGAU-AGGGUCUCCUU--------AGCAG- -5'
32839 3' -49 NC_007346.1 + 342699 1.09 0.032115
Target:  5'- aACUGAUACUAUCCCAGAGGAAUCGUCc -3'
miRNA:   3'- -UGACUAUGAUAGGGUCUCCUUAGCAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.