Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32839 | 3' | -49 | NC_007346.1 | + | 342699 | 1.09 | 0.032115 |
Target: 5'- aACUGAUACUAUCCCAGAGGAAUCGUCc -3' miRNA: 3'- -UGACUAUGAUAGGGUCUCCUUAGCAG- -5' |
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32839 | 3' | -49 | NC_007346.1 | + | 188147 | 0.73 | 0.986483 |
Target: 5'- cCUGAUGCUAgUUCUGGGGGAAgugaaguuUCGUCa -3' miRNA: 3'- uGACUAUGAU-AGGGUCUCCUU--------AGCAG- -5' |
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32839 | 3' | -49 | NC_007346.1 | + | 7993 | 0.7 | 0.99897 |
Target: 5'- --aGAUugUGaCCCGGcgucGGAAUCGUCa -3' miRNA: 3'- ugaCUAugAUaGGGUCu---CCUUAGCAG- -5' |
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32839 | 3' | -49 | NC_007346.1 | + | 171885 | 0.68 | 0.999811 |
Target: 5'- aGCUGAUACUucuuuUCCUAacaauGGAAUgCGUCg -3' miRNA: 3'- -UGACUAUGAu----AGGGUcu---CCUUA-GCAG- -5' |
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32839 | 3' | -49 | NC_007346.1 | + | 177999 | 0.67 | 0.99997 |
Target: 5'- cACUGGUGgUAUCCUAGGu--AUUGUCa -3' miRNA: 3'- -UGACUAUgAUAGGGUCUccuUAGCAG- -5' |
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32839 | 3' | -49 | NC_007346.1 | + | 309576 | 0.66 | 0.999994 |
Target: 5'- cACUG--GCUcgUCaCGGAGGcAUCGUCg -3' miRNA: 3'- -UGACuaUGAuaGG-GUCUCCuUAGCAG- -5' |
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32839 | 3' | -49 | NC_007346.1 | + | 223940 | 0.66 | 0.999983 |
Target: 5'- uACUGGagGCggggAUUCCGGGGGGAUgGUa -3' miRNA: 3'- -UGACUa-UGa---UAGGGUCUCCUUAgCAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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