Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32841 | 5' | -52 | NC_007346.1 | + | 401646 | 0.67 | 0.997853 |
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Target: 5'- uUUUGuuUCUgCGCGGUUGCCuCACUUGc -3' miRNA: 3'- -AAACu-AGA-GCGCCAAUGGcGUGGAUu -5' |
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| 32841 | 5' | -52 | NC_007346.1 | + | 17899 | 0.67 | 0.99651 |
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Target: 5'- --aGAaCUUGCGGUUGCUGUACguCUAAa -3' miRNA: 3'- aaaCUaGAGCGCCAAUGGCGUG--GAUU- -5' |
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| 32841 | 5' | -52 | NC_007346.1 | + | 326659 | 0.68 | 0.995278 |
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Target: 5'- gUUGAUUcaacuUUGCGGgaGCUGCACCa-- -3' miRNA: 3'- aAACUAG-----AGCGCCaaUGGCGUGGauu -5' |
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| 32841 | 5' | -52 | NC_007346.1 | + | 313241 | 0.68 | 0.994846 |
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Target: 5'- gUUGGUgUCGCGGUuguauuaauuauaguUACCGCAaCCc-- -3' miRNA: 3'- aAACUAgAGCGCCA---------------AUGGCGU-GGauu -5' |
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| 32841 | 5' | -52 | NC_007346.1 | + | 148846 | 0.7 | 0.980927 |
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Target: 5'- --aGAUUaCGUcGUUGCCGCGCCUAu -3' miRNA: 3'- aaaCUAGaGCGcCAAUGGCGUGGAUu -5' |
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| 32841 | 5' | -52 | NC_007346.1 | + | 384633 | 1.04 | 0.027871 |
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Target: 5'- aUUUGAUCUCGCGGUUACCGCACCUAAg -3' miRNA: 3'- -AAACUAGAGCGCCAAUGGCGUGGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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