Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32843 | 5' | -59.4 | NC_007346.1 | + | 87523 | 0.66 | 0.916755 |
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Target: 5'- cAGC-AGUCGUucGCGUCGCUCGACAAc -3' miRNA: 3'- -UUGuUCGGCGccCGCGGCGAGUUGUU- -5' |
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| 32843 | 5' | -59.4 | NC_007346.1 | + | 363511 | 0.66 | 0.911222 |
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Target: 5'- uAACAuauGCUGCGGGCGU---UCAACAAu -3' miRNA: 3'- -UUGUu--CGGCGCCCGCGgcgAGUUGUU- -5' |
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| 32843 | 5' | -59.4 | NC_007346.1 | + | 207452 | 0.66 | 0.905479 |
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Target: 5'- uGCAAGaCUGCGuGGCGUCGgUCaAACAu -3' miRNA: 3'- uUGUUC-GGCGC-CCGCGGCgAG-UUGUu -5' |
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| 32843 | 5' | -59.4 | NC_007346.1 | + | 44148 | 0.66 | 0.893371 |
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Target: 5'- gGACAAGCaGCGGcgcGCGCUGCUgCGAUg- -3' miRNA: 3'- -UUGUUCGgCGCC---CGCGGCGA-GUUGuu -5' |
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| 32843 | 5' | -59.4 | NC_007346.1 | + | 98270 | 0.75 | 0.461639 |
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Target: 5'- gAACGGGCuCGCaGGCGCCGC-CGGCGGu -3' miRNA: 3'- -UUGUUCG-GCGcCCGCGGCGaGUUGUU- -5' |
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| 32843 | 5' | -59.4 | NC_007346.1 | + | 401131 | 1.03 | 0.007335 |
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Target: 5'- cAACAAGCCGCGGGCGCCGCUCAACAAc -3' miRNA: 3'- -UUGUUCGGCGCCCGCGGCGAGUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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