Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32850 | 3' | -51.2 | NC_007383.1 | + | 43140 | 0.68 | 0.97014 |
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Target: 5'- gGCUGAGgUCAUCGUcgGAGgUUAUCGa -3' miRNA: 3'- gCGACUUgGGUAGCAa-CUUgGGUAGC- -5' |
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| 32850 | 3' | -51.2 | NC_007383.1 | + | 125505 | 0.68 | 0.97014 |
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Target: 5'- gGCUGAGgUCAUCGUcgGAGgUUAUCGa -3' miRNA: 3'- gCGACUUgGGUAGCAa-CUUgGGUAGC- -5' |
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| 32850 | 3' | -51.2 | NC_007383.1 | + | 3941 | 0.78 | 0.544208 |
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Target: 5'- aCGCUGAGCCCGaCGcUGAACCCg--- -3' miRNA: 3'- -GCGACUUGGGUaGCaACUUGGGuagc -5' |
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| 32850 | 3' | -51.2 | NC_007383.1 | + | 3892 | 0.78 | 0.53393 |
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Target: 5'- nGCUGAGCCCGaCGcUGAGCCCGagGa -3' miRNA: 3'- gCGACUUGGGUaGCaACUUGGGUagC- -5' |
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| 32850 | 3' | -51.2 | NC_007383.1 | + | 3988 | 0.88 | 0.165529 |
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Target: 5'- nGCUGAGCCCGaCGcUGAACCCGUCGc -3' miRNA: 3'- gCGACUUGGGUaGCaACUUGGGUAGC- -5' |
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| 32850 | 3' | -51.2 | NC_007383.1 | + | 3917 | 1 | 0.030767 |
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Target: 5'- aCGCUGAACCCGUCGcUGAGCCCGUCGc -3' miRNA: 3'- -GCGACUUGGGUAGCaACUUGGGUAGC- -5' |
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| 32850 | 3' | -51.2 | NC_007383.1 | + | 4013 | 1.09 | 0.00738 |
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Target: 5'- aCGCUGAACCCAUCGUUGAACCCAUCGc -3' miRNA: 3'- -GCGACUUGGGUAGCAACUUGGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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