Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32850 | 5' | -56.6 | NC_007383.1 | + | 104812 | 0.69 | 0.728059 |
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Target: 5'- aACGCUuuACCCGUCGCaaugcUGGGCaUGAa -3' miRNA: 3'- cUGCGAcuUGGGCAGCG-----ACUCGaGCU- -5' |
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| 32850 | 5' | -56.6 | NC_007383.1 | + | 4219 | 0.69 | 0.728059 |
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Target: 5'- uGAgGCUGAGCCUGaaucugaaGCUGAGCcUGAa -3' miRNA: 3'- -CUgCGACUUGGGCag------CGACUCGaGCU- -5' |
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| 32850 | 5' | -56.6 | NC_007383.1 | + | 4015 | 0.73 | 0.46357 |
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Target: 5'- cGACGCUGAACCCaucguugaacccaUCGCUGAGCc--- -3' miRNA: 3'- -CUGCGACUUGGGc------------AGCGACUCGagcu -5' |
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| 32850 | 5' | -56.6 | NC_007383.1 | + | 3954 | 0.81 | 0.164027 |
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Target: 5'- nACGCUGAGCUCGaCGCUGAGCcCGAc -3' miRNA: 3'- cUGCGACUUGGGCaGCGACUCGaGCU- -5' |
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| 32850 | 5' | -56.6 | NC_007383.1 | + | 3893 | 0.84 | 0.105441 |
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Target: 5'- -uCGCUGAGCCCGaCGCUGAGCcCGAg -3' miRNA: 3'- cuGCGACUUGGGCaGCGACUCGaGCU- -5' |
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| 32850 | 5' | -56.6 | NC_007383.1 | + | 3919 | 0.96 | 0.016997 |
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Target: 5'- cGACGCUGAACCCGUCGCUGAGCcCGu -3' miRNA: 3'- -CUGCGACUUGGGCAGCGACUCGaGCu -5' |
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| 32850 | 5' | -56.6 | NC_007383.1 | + | 3979 | 1.09 | 0.002356 |
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Target: 5'- cGACGCUGAACCCGUCGCUGAGCUCGAc -3' miRNA: 3'- -CUGCGACUUGGGCAGCGACUCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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