Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32852 | 5' | -45.2 | NC_007383.1 | + | 60502 | 0.66 | 0.999995 |
Target: 5'- aGUCGAUGgcCGUGUUG---GUCGAAGa -3' miRNA: 3'- aCAGCUAU--GCACGAUaagCAGUUUCc -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 54629 | 0.66 | 0.999993 |
Target: 5'- aGUCGAccaAC-UGCUGUUCGg-AAAGGu -3' miRNA: 3'- aCAGCUa--UGcACGAUAAGCagUUUCC- -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 119223 | 0.67 | 0.999973 |
Target: 5'- aUGUCG--GCGUGUUcgUUGUCAucGa -3' miRNA: 3'- -ACAGCuaUGCACGAuaAGCAGUuuCc -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 133479 | 0.67 | 0.999951 |
Target: 5'- aUGUCGu--CGUGUUAUUUGcUCAAcauccAGGa -3' miRNA: 3'- -ACAGCuauGCACGAUAAGC-AGUU-----UCC- -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 73358 | 0.68 | 0.999912 |
Target: 5'- cGUCGAUGauuucGUUGUaUGUCAGAGGu -3' miRNA: 3'- aCAGCUAUgca--CGAUAaGCAGUUUCC- -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 110581 | 0.69 | 0.999486 |
Target: 5'- aUGUCGAUGcCGUcCUcgUCGUCAGc-- -3' miRNA: 3'- -ACAGCUAU-GCAcGAuaAGCAGUUucc -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 43126 | 0.69 | 0.999486 |
Target: 5'- cGUCagccgGAUGCG-GCUGaggucaUCGUCGGAGGu -3' miRNA: 3'- aCAG-----CUAUGCaCGAUa-----AGCAGUUUCC- -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 125519 | 0.69 | 0.999486 |
Target: 5'- cGUCagccgGAUGCG-GCUGaggucaUCGUCGGAGGu -3' miRNA: 3'- aCAG-----CUAUGCaCGAUa-----AGCAGUUUCC- -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 123125 | 0.71 | 0.996348 |
Target: 5'- aGUgGAUGCGUGUcaagUGUUCGUCGc--- -3' miRNA: 3'- aCAgCUAUGCACG----AUAAGCAGUuucc -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 105432 | 0.74 | 0.98438 |
Target: 5'- gUGUCGAcGCaaGUGCUGUucgagcaaUCGUCAAAGu -3' miRNA: 3'- -ACAGCUaUG--CACGAUA--------AGCAGUUUCc -5' |
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32852 | 5' | -45.2 | NC_007383.1 | + | 34055 | 1.13 | 0.018714 |
Target: 5'- cUGUCGAUACGUGCUAUUCGUCAAAGGa -3' miRNA: 3'- -ACAGCUAUGCACGAUAAGCAGUUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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