Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32853 | 3' | -60 | NC_007383.1 | + | 65351 | 0.66 | 0.758882 |
Target: 5'- gACGUcgaacuuuugGUCGGCACGGaCGaCGAUGUCGu -3' miRNA: 3'- -UGCG----------UAGCUGUGCC-GCgGCUGCGGCu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 107951 | 0.66 | 0.730602 |
Target: 5'- -aGCAgUCGAauuuaAUGuCGCCGACGUCGAa -3' miRNA: 3'- ugCGU-AGCUg----UGCcGCGGCUGCGGCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 80848 | 0.67 | 0.705497 |
Target: 5'- cCGC-UCGAC-CGGCGUcucccuccgcuagaaCGACGCCu- -3' miRNA: 3'- uGCGuAGCUGuGCCGCG---------------GCUGCGGcu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 56419 | 0.67 | 0.691805 |
Target: 5'- uCGCGuUCGGCAU--CGCCGACGuuGGa -3' miRNA: 3'- uGCGU-AGCUGUGccGCGGCUGCggCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 115576 | 0.69 | 0.572881 |
Target: 5'- gGCGUA-CGACAacucCGGCccuGCUGGCGCCGc -3' miRNA: 3'- -UGCGUaGCUGU----GCCG---CGGCUGCGGCu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 83686 | 0.69 | 0.572881 |
Target: 5'- cGCGUGUCGAUAuCGGUGCCGuCGauaGAa -3' miRNA: 3'- -UGCGUAGCUGU-GCCGCGGCuGCgg-CU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 23721 | 0.69 | 0.56307 |
Target: 5'- -gGCAUCGGCggcggaggugGCGGCGauGAUGUCGAc -3' miRNA: 3'- ugCGUAGCUG----------UGCCGCggCUGCGGCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 44337 | 0.69 | 0.553305 |
Target: 5'- -gGCGUCGGCGCcGUGUCGAUGCgUGAu -3' miRNA: 3'- ugCGUAGCUGUGcCGCGGCUGCG-GCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 86014 | 0.7 | 0.524338 |
Target: 5'- aACGCuaaauaCG-CGCGuuuCGCCGACGCCGAc -3' miRNA: 3'- -UGCGua----GCuGUGCc--GCGGCUGCGGCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 3954 | 0.7 | 0.495968 |
Target: 5'- gACGCugagcUCGACGCuGaGC-CCGACGCUGAa -3' miRNA: 3'- -UGCGu----AGCUGUG-C-CGcGGCUGCGGCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 43405 | 0.71 | 0.450322 |
Target: 5'- gAUGUggCGGCGgaucucuuCGGCGUCGGCGCCGu -3' miRNA: 3'- -UGCGuaGCUGU--------GCCGCGGCUGCGGCu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 125241 | 0.71 | 0.450322 |
Target: 5'- gAUGUggCGGCGgaucucuuCGGCGUCGGCGCCGu -3' miRNA: 3'- -UGCGuaGCUGU--------GCCGCGGCUGCGGCu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 80949 | 0.72 | 0.415523 |
Target: 5'- -gGCGUCGcucaaGCGGCgGCCGACGUCGc -3' miRNA: 3'- ugCGUAGCug---UGCCG-CGGCUGCGGCu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 55095 | 0.74 | 0.300843 |
Target: 5'- gGCGCugcugcgacauaGUCGAUaACGGCGuuGACGCCa- -3' miRNA: 3'- -UGCG------------UAGCUG-UGCCGCggCUGCGGcu -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 43583 | 0.97 | 0.00829 |
Target: 5'- cACGCAU-GACACGGCGCCGACGCCGAa -3' miRNA: 3'- -UGCGUAgCUGUGCCGCGGCUGCGGCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 125062 | 1.08 | 0.001433 |
Target: 5'- cACGCAUCGACACGGCGCCGACGCCGAa -3' miRNA: 3'- -UGCGUAGCUGUGCCGCGGCUGCGGCU- -5' |
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32853 | 3' | -60 | NC_007383.1 | + | 44179 | 1.08 | 0.001433 |
Target: 5'- cACGCAUCGACACGGCGCCGACGCCGAa -3' miRNA: 3'- -UGCGUAGCUGUGCCGCGGCUGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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