Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32853 | 5' | -53.6 | NC_007383.1 | + | 95177 | 0.66 | 0.967624 |
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Target: 5'- uCGCCGCGgaGGCGUGaugcacgauaauCGGUuugaucaaCGAUUCAu -3' miRNA: 3'- -GCGGUGUagCCGCAU------------GCCA--------GCUAAGU- -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 131670 | 0.66 | 0.960914 |
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Target: 5'- -cCCACGuagcauguuaauUCGGCGUcguCGGUCGAUg-- -3' miRNA: 3'- gcGGUGU------------AGCCGCAu--GCCAGCUAagu -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 66254 | 0.66 | 0.95607 |
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Target: 5'- uGCCAgGUCGGUGUcguuaccguugacaACGG-CGGUaUCGa -3' miRNA: 3'- gCGGUgUAGCCGCA--------------UGCCaGCUA-AGU- -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 81874 | 0.68 | 0.913228 |
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Target: 5'- uCGCCGuCGUCGGU--GCGGUCG--UCGc -3' miRNA: 3'- -GCGGU-GUAGCCGcaUGCCAGCuaAGU- -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 81753 | 0.69 | 0.880282 |
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Target: 5'- gGCCAUGUUGGCGUuagagcuuuCGGUCG--UCGu -3' miRNA: 3'- gCGGUGUAGCCGCAu--------GCCAGCuaAGU- -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 84433 | 0.69 | 0.871509 |
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Target: 5'- cCGCCAcCGUCGGUGgcagcguacuuCGGUCGAa--- -3' miRNA: 3'- -GCGGU-GUAGCCGCau---------GCCAGCUaagu -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 44129 | 0.77 | 0.448839 |
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Target: 5'- aGUCGCAuaguguUCGauuuaaGCGUACGGUCGAUUCAu -3' miRNA: 3'- gCGGUGU------AGC------CGCAUGCCAGCUAAGU- -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 125098 | 1.09 | 0.004754 |
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Target: 5'- cCGCCACAUCGGCGUACGGUCGAUUCAu -3' miRNA: 3'- -GCGGUGUAGCCGCAUGCCAGCUAAGU- -5' |
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| 32853 | 5' | -53.6 | NC_007383.1 | + | 43548 | 1.09 | 0.004754 |
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Target: 5'- cCGCCACAUCGGCGUACGGUCGAUUCAu -3' miRNA: 3'- -GCGGUGUAGCCGCAUGCCAGCUAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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