Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32854 | 3' | -48.9 | NC_007383.1 | + | 11449 | 0.73 | 0.887362 |
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Target: 5'- aCAACGAUGACGaUGACGACAAaGAa- -3' miRNA: 3'- -GUUGCUGCUGUcGCUGCUGUUaCUau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 38693 | 0.73 | 0.901361 |
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Target: 5'- uGACGACGACGaCGAauauacCGACGAUGAUu -3' miRNA: 3'- gUUGCUGCUGUcGCU------GCUGUUACUAu -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 27066 | 0.72 | 0.92039 |
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Target: 5'- gCAACGAaaaugguaaaaaUGGCAGCGAaGACGAUGAc- -3' miRNA: 3'- -GUUGCU------------GCUGUCGCUgCUGUUACUau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 39939 | 0.72 | 0.92039 |
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Target: 5'- aCGAaGACGAgGGCGACGACAAg---- -3' miRNA: 3'- -GUUgCUGCUgUCGCUGCUGUUacuau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 5511 | 0.72 | 0.922746 |
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Target: 5'- uGACGACGACauaagugauaaaaguGGUGACGACGAUa--- -3' miRNA: 3'- gUUGCUGCUG---------------UCGCUGCUGUUAcuau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 64426 | 1.08 | 0.014818 |
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Target: 5'- cCAACGACGACAGCGACGACAAUGAUAa -3' miRNA: 3'- -GUUGCUGCUGUCGCUGCUGUUACUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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