Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32854 | 3' | -48.9 | NC_007383.1 | + | 127113 | 0.78 | 0.648111 |
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Target: 5'- aCGACGACGAUAGCGACaaaGAUGAa- -3' miRNA: 3'- -GUUGCUGCUGUCGCUGcugUUACUau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 36955 | 0.82 | 0.458902 |
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Target: 5'- aGACGAUgaaGGCGGCGGCGACAGUGGc- -3' miRNA: 3'- gUUGCUG---CUGUCGCUGCUGUUACUau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 23725 | 0.82 | 0.439523 |
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Target: 5'- uCGGCGGCGGaGGUGGCGGCGAUGAUGu -3' miRNA: 3'- -GUUGCUGCUgUCGCUGCUGUUACUAU- -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 15578 | 0.87 | 0.241892 |
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Target: 5'- aCAGCGACaACAGCGAUGACGAUGAa- -3' miRNA: 3'- -GUUGCUGcUGUCGCUGCUGUUACUau -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 45415 | 0.91 | 0.158641 |
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Target: 5'- aCGACGACGACGaCGACGGCAGUGAUGa -3' miRNA: 3'- -GUUGCUGCUGUcGCUGCUGUUACUAU- -5' |
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| 32854 | 3' | -48.9 | NC_007383.1 | + | 64426 | 1.08 | 0.014818 |
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Target: 5'- cCAACGACGACAGCGACGACAAUGAUAa -3' miRNA: 3'- -GUUGCUGCUGUCGCUGCUGUUACUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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