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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32854 | 5' | -44.8 | NC_007383.1 | + | 81859 | 0.67 | 0.999945 |
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Target: 5'- --gAAAAGgacaCGAA-GUCGCCGUCGUc -3' miRNA: 3'- uuaUUUUCa---GUUUgCAGCGGUAGCAa -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 7369 | 0.67 | 0.9999 |
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Target: 5'- --gAGGAGUCAGACGa-GCCgGUCGUc -3' miRNA: 3'- uuaUUUUCAGUUUGCagCGG-UAGCAa -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 51700 | 0.69 | 0.999246 |
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Target: 5'- --cAGucGUCGuuguCGUCGUCAUCGUUg -3' miRNA: 3'- uuaUUuuCAGUuu--GCAGCGGUAGCAA- -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 16919 | 0.69 | 0.999246 |
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Target: 5'- -uUGAAcAGUUuauuGACGUCGCUAUCGg- -3' miRNA: 3'- uuAUUU-UCAGu---UUGCAGCGGUAGCaa -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 20471 | 0.69 | 0.999062 |
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Target: 5'- --aAAGAcGUCGAugGCGUUGCCGUUGUc -3' miRNA: 3'- uuaUUUU-CAGUU--UGCAGCGGUAGCAa -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 66995 | 0.7 | 0.998574 |
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Target: 5'- -uUAAuAGUCGAuucUGUUGCCGUCGUc -3' miRNA: 3'- uuAUUuUCAGUUu--GCAGCGGUAGCAa -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 32619 | 0.77 | 0.905531 |
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Target: 5'- ------uGUCGucGGCGUCGCCAUCGUa -3' miRNA: 3'- uuauuuuCAGU--UUGCAGCGGUAGCAa -5' |
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| 32854 | 5' | -44.8 | NC_007383.1 | + | 64392 | 1.04 | 0.052612 |
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Target: 5'- uAAUAAAAGUCAAACGUCGCCAUCGUUg -3' miRNA: 3'- -UUAUUUUCAGUUUGCAGCGGUAGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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