Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32855 | 5' | -58 | NC_007383.1 | + | 119732 | 0.66 | 0.843101 |
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Target: 5'- uGUUCCCaacgCCGGCCuACaGUcAUCaGGCg -3' miRNA: 3'- gCAAGGGa---GGCCGGuUG-CGuUGG-CCG- -5' |
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| 32855 | 5' | -58 | NC_007383.1 | + | 103726 | 0.66 | 0.818395 |
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Target: 5'- --gUCn-UCCGGCgGACGUAGgcgcucauCCGGCg -3' miRNA: 3'- gcaAGggAGGCCGgUUGCGUU--------GGCCG- -5' |
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| 32855 | 5' | -58 | NC_007383.1 | + | 52592 | 0.67 | 0.795781 |
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Target: 5'- aGUUCaaggCCGGUCGACGCAucuucaaagaaaaCGGCa -3' miRNA: 3'- gCAAGgga-GGCCGGUUGCGUug-----------GCCG- -5' |
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| 32855 | 5' | -58 | NC_007383.1 | + | 87875 | 0.69 | 0.677213 |
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Target: 5'- aGUUUCCaUCCGGCCuuUGCAuACaCGGg -3' miRNA: 3'- gCAAGGG-AGGCCGGuuGCGU-UG-GCCg -5' |
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| 32855 | 5' | -58 | NC_007383.1 | + | 52539 | 0.71 | 0.566728 |
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Target: 5'- uGUaUUCUCUGGUgAGCGUAcuGCCGGCg -3' miRNA: 3'- gCAaGGGAGGCCGgUUGCGU--UGGCCG- -5' |
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| 32855 | 5' | -58 | NC_007383.1 | + | 69404 | 1.12 | 0.001129 |
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Target: 5'- cCGUUCCCUCCGGCCAACGCAACCGGCg -3' miRNA: 3'- -GCAAGGGAGGCCGGUUGCGUUGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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