Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32856 | 5' | -46.4 | NC_007383.1 | + | 54989 | 0.67 | 0.999595 |
Target: 5'- cCGAUaaucacgUUCGUUGUCGUCGgCAuAUACu- -3' miRNA: 3'- -GCUG-------AAGCAACAGCAGCaGU-UAUGuu -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 126824 | 0.68 | 0.999377 |
Target: 5'- uCGAUUgUUGUucUGUCGUUGUCGGUAUg- -3' miRNA: 3'- -GCUGA-AGCA--ACAGCAGCAGUUAUGuu -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 51701 | 0.7 | 0.99579 |
Target: 5'- aGuCgUCGUUGUCGUCGUCAu----- -3' miRNA: 3'- gCuGaAGCAACAGCAGCAGUuauguu -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 42449 | 0.71 | 0.992194 |
Target: 5'- cCGACUuuaacauuccagUCGUUGUaGUCGUCGGguugGCGAa -3' miRNA: 3'- -GCUGA------------AGCAACAgCAGCAGUUa---UGUU- -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 64552 | 0.72 | 0.98266 |
Target: 5'- cCGugUUUuUUGUCGUaGUCAAUGCAGc -3' miRNA: 3'- -GCugAAGcAACAGCAgCAGUUAUGUU- -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 107860 | 0.76 | 0.906067 |
Target: 5'- uCGGUUUCGUUGUCGUCGaCAuUACAGg -3' miRNA: 3'- -GCUGAAGCAACAGCAGCaGUuAUGUU- -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 62941 | 0.77 | 0.861405 |
Target: 5'- uCGuCUUCGUUGUCGUCGUCuucguCAu -3' miRNA: 3'- -GCuGAAGCAACAGCAGCAGuuau-GUu -5' |
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32856 | 5' | -46.4 | NC_007383.1 | + | 81990 | 1.08 | 0.027731 |
Target: 5'- gCGACUUCGUUGUCGUCGUCAAUACAAa -3' miRNA: 3'- -GCUGAAGCAACAGCAGCAGUUAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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