miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32857 3' -51.9 NC_007383.1 + 131940 0.66 0.985938
Target:  5'- --cGGUCUUGaAGAGCgccuugaCGUCGUGUGg -3'
miRNA:   3'- ggaCUAGAAU-UCUCGa------GCGGCGCACg -5'
32857 3' -51.9 NC_007383.1 + 59827 0.66 0.977673
Target:  5'- aCUGGaugugGAGGGaUUGUCGCGUGCa -3'
miRNA:   3'- gGACUagaa-UUCUCgAGCGGCGCACG- -5'
32857 3' -51.9 NC_007383.1 + 80650 0.67 0.966285
Target:  5'- uCCUGAgg--AAGAGCgucUGCCGCGa-- -3'
miRNA:   3'- -GGACUagaaUUCUCGa--GCGGCGCacg -5'
32857 3' -51.9 NC_007383.1 + 63551 0.67 0.962893
Target:  5'- cUCUGAUaUUUuuGAGCUCuuuGUCGCGUGg -3'
miRNA:   3'- -GGACUA-GAAuuCUCGAG---CGGCGCACg -5'
32857 3' -51.9 NC_007383.1 + 63849 0.68 0.955412
Target:  5'- gUUGAUCccgAAGAGCugugUCGUCGCaUGCu -3'
miRNA:   3'- gGACUAGaa-UUCUCG----AGCGGCGcACG- -5'
32857 3' -51.9 NC_007383.1 + 94140 1.14 0.003178
Target:  5'- uCCUGAUCUUAAGAGCUCGCCGCGUGCu -3'
miRNA:   3'- -GGACUAGAAUUCUCGAGCGGCGCACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.