Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
32857 | 3' | -51.9 | NC_007383.1 | + | 131940 | 0.66 | 0.985938 |
Target: 5'- --cGGUCUUGaAGAGCgccuugaCGUCGUGUGg -3' miRNA: 3'- ggaCUAGAAU-UCUCGa------GCGGCGCACg -5' |
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32857 | 3' | -51.9 | NC_007383.1 | + | 59827 | 0.66 | 0.977673 |
Target: 5'- aCUGGaugugGAGGGaUUGUCGCGUGCa -3' miRNA: 3'- gGACUagaa-UUCUCgAGCGGCGCACG- -5' |
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32857 | 3' | -51.9 | NC_007383.1 | + | 80650 | 0.67 | 0.966285 |
Target: 5'- uCCUGAgg--AAGAGCgucUGCCGCGa-- -3' miRNA: 3'- -GGACUagaaUUCUCGa--GCGGCGCacg -5' |
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32857 | 3' | -51.9 | NC_007383.1 | + | 63551 | 0.67 | 0.962893 |
Target: 5'- cUCUGAUaUUUuuGAGCUCuuuGUCGCGUGg -3' miRNA: 3'- -GGACUA-GAAuuCUCGAG---CGGCGCACg -5' |
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32857 | 3' | -51.9 | NC_007383.1 | + | 63849 | 0.68 | 0.955412 |
Target: 5'- gUUGAUCccgAAGAGCugugUCGUCGCaUGCu -3' miRNA: 3'- gGACUAGaa-UUCUCG----AGCGGCGcACG- -5' |
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32857 | 3' | -51.9 | NC_007383.1 | + | 94140 | 1.14 | 0.003178 |
Target: 5'- uCCUGAUCUUAAGAGCUCGCCGCGUGCu -3' miRNA: 3'- -GGACUAGAAUUCUCGAGCGGCGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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