Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32859 | 5' | -48.6 | NC_007383.1 | + | 63852 | 0.66 | 0.998514 |
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Target: 5'- aUCGUUGAUcCCGAagagcugUGUCGuCGCAUgCUCg -3' miRNA: 3'- gAGCAGUUA-GGCU-------ACAGU-GUGUA-GAG- -5' |
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| 32859 | 5' | -48.6 | NC_007383.1 | + | 81879 | 0.66 | 0.997881 |
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Target: 5'- gUCGUCGGUgCGGUcGUCGCuccuCcUCUCu -3' miRNA: 3'- gAGCAGUUAgGCUA-CAGUGu---GuAGAG- -5' |
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| 32859 | 5' | -48.6 | NC_007383.1 | + | 84642 | 0.67 | 0.996432 |
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Target: 5'- uUCGUuuaaaCGAUgCCGAUGUCGuCGCGUCcaUCg -3' miRNA: 3'- gAGCA-----GUUA-GGCUACAGU-GUGUAG--AG- -5' |
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| 32859 | 5' | -48.6 | NC_007383.1 | + | 53676 | 0.71 | 0.960007 |
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Target: 5'- aCUCuUCGgcuaguagcAUCCGAUGUCGCugAUCa- -3' miRNA: 3'- -GAGcAGU---------UAGGCUACAGUGugUAGag -5' |
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| 32859 | 5' | -48.6 | NC_007383.1 | + | 61979 | 0.74 | 0.888567 |
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Target: 5'- gUCGUCAAUCCGucagcaGUGUCACAUuAUC-Ca -3' miRNA: 3'- gAGCAGUUAGGC------UACAGUGUG-UAGaG- -5' |
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| 32859 | 5' | -48.6 | NC_007383.1 | + | 94885 | 1.11 | 0.009631 |
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Target: 5'- uCUCGUCAAUCCGAUGUCACACAUCUCg -3' miRNA: 3'- -GAGCAGUUAGGCUACAGUGUGUAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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