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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32860 | 5' | -59.5 | NC_007383.1 | + | 22558 | 0.67 | 0.703408 |
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Target: 5'- cACCGCCGGAgcucUGAcCGCCgucgAUGccUCCGa -3' miRNA: 3'- cUGGCGGCCU----ACUcGCGGa---UGC--AGGC- -5' |
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| 32860 | 5' | -59.5 | NC_007383.1 | + | 80642 | 0.72 | 0.40685 |
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Target: 5'- cGACUGCCuccugaGGAaGAGCGUCUgccgcgaggACGUCCGg -3' miRNA: 3'- -CUGGCGG------CCUaCUCGCGGA---------UGCAGGC- -5' |
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| 32860 | 5' | -59.5 | NC_007383.1 | + | 80795 | 0.72 | 0.390191 |
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Target: 5'- cGGCCGCCGcuUGAGCgacGCCUAuucUGUCCGc -3' miRNA: 3'- -CUGGCGGCcuACUCG---CGGAU---GCAGGC- -5' |
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| 32860 | 5' | -59.5 | NC_007383.1 | + | 103957 | 0.76 | 0.23227 |
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Target: 5'- cGGCCGCCaGAUGAaCGCCUACGaccUCCa -3' miRNA: 3'- -CUGGCGGcCUACUcGCGGAUGC---AGGc -5' |
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| 32860 | 5' | -59.5 | NC_007383.1 | + | 103894 | 1.08 | 0.001619 |
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Target: 5'- cGACCGCCGGAUGAGCGCCUACGUCCGc -3' miRNA: 3'- -CUGGCGGCCUACUCGCGGAUGCAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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