Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32861 | 3' | -50.4 | NC_007383.1 | + | 75078 | 0.66 | 0.995214 |
Target: 5'- --aGGAUCGGgaCG-ACGacCCGAUGUGGg -3' miRNA: 3'- guaCUUAGCUg-GCaUGC--GGCUACACC- -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 52970 | 0.67 | 0.992563 |
Target: 5'- --cGGAUCGaACCGUGuCGCCGAc---- -3' miRNA: 3'- guaCUUAGC-UGGCAU-GCGGCUacacc -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 34002 | 0.67 | 0.991339 |
Target: 5'- -cUGAAUCGugCGUuauaccaGCGCCGAa---- -3' miRNA: 3'- guACUUAGCugGCA-------UGCGGCUacacc -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 43460 | 0.67 | 0.990224 |
Target: 5'- uCAUGGAUCGACCGUAgGagGAuuUGUa- -3' miRNA: 3'- -GUACUUAGCUGGCAUgCggCU--ACAcc -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 125186 | 0.67 | 0.990224 |
Target: 5'- uCAUGGAUCGACCGUAgGagGAuuUGUa- -3' miRNA: 3'- -GUACUUAGCUGGCAUgCggCU--ACAcc -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 37450 | 0.68 | 0.979669 |
Target: 5'- --aGAG-CGAUCGUACGCUauaguGAUGUGa -3' miRNA: 3'- guaCUUaGCUGGCAUGCGG-----CUACACc -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 79439 | 0.68 | 0.977299 |
Target: 5'- aAUGAAccagacuaaacUCGAgCCGUAUuUCGAUGUGGu -3' miRNA: 3'- gUACUU-----------AGCU-GGCAUGcGGCUACACC- -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 105300 | 0.75 | 0.759806 |
Target: 5'- aCAUGcAGUCGGCCGUGC-CCGAaaUGGa -3' miRNA: 3'- -GUAC-UUAGCUGGCAUGcGGCUacACC- -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 44303 | 0.77 | 0.67814 |
Target: 5'- uCAUGAAUCGACCGUACGCUu------ -3' miRNA: 3'- -GUACUUAGCUGGCAUGCGGcuacacc -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 43385 | 1.1 | 0.009078 |
Target: 5'- uCAUGAAUCGACCGUACGCCGAUGUGGc -3' miRNA: 3'- -GUACUUAGCUGGCAUGCGGCUACACC- -5' |
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32861 | 3' | -50.4 | NC_007383.1 | + | 125261 | 1.1 | 0.009078 |
Target: 5'- uCAUGAAUCGACCGUACGCCGAUGUGGc -3' miRNA: 3'- -GUACUUAGCUGGCAUGCGGCUACACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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