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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32863 | 3' | -45.2 | NC_007383.1 | + | 32436 | 0.66 | 0.999968 |
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Target: 5'- uUGCUGUGccgUCGACGuucaaggaagaugcGUGCUGcacaaaugUGUACu -3' miRNA: 3'- -ACGAUAUa--AGCUGC--------------CACGAUa-------ACAUG- -5' |
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| 32863 | 3' | -45.2 | NC_007383.1 | + | 128120 | 0.68 | 0.999639 |
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Target: 5'- aUGCUcacg-CG-CGGcgGCUAUUGUACa -3' miRNA: 3'- -ACGAuauaaGCuGCCa-CGAUAACAUG- -5' |
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| 32863 | 3' | -45.2 | NC_007383.1 | + | 38738 | 0.7 | 0.997086 |
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Target: 5'- cUGCUuugAUAUuaUCGACGGUaCUgAUUGUGCu -3' miRNA: 3'- -ACGA---UAUA--AGCUGCCAcGA-UAACAUG- -5' |
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| 32863 | 3' | -45.2 | NC_007383.1 | + | 26693 | 0.71 | 0.99588 |
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Target: 5'- aGCccgAUAa--GGCGGUGCUGcUGUGCg -3' miRNA: 3'- aCGa--UAUaagCUGCCACGAUaACAUG- -5' |
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| 32863 | 3' | -45.2 | NC_007383.1 | + | 26497 | 1.13 | 0.016351 |
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Target: 5'- uUGCUAUAUUCGACGGUGCUAUUGUACg -3' miRNA: 3'- -ACGAUAUAAGCUGCCACGAUAACAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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