Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32863 | 5' | -56 | NC_007383.1 | + | 12929 | 0.66 | 0.901068 |
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Target: 5'- aGCGCcgGCGGCGGUaguucuccagcaaACgCGCCUccagCGGGc -3' miRNA: 3'- -CGCG--UGUCGUCG-------------UG-GCGGAaua-GCCC- -5' |
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| 32863 | 5' | -56 | NC_007383.1 | + | 65159 | 0.66 | 0.874344 |
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Target: 5'- cUGCACucgAGCAGCgucgaagaaGCCGCCcgucaAUUGGGa -3' miRNA: 3'- cGCGUG---UCGUCG---------UGGCGGaa---UAGCCC- -5' |
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| 32863 | 5' | -56 | NC_007383.1 | + | 17818 | 0.66 | 0.874344 |
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Target: 5'- uGCGCcuucuCGcGUAGcCGCCGCCcUGUCGaGGu -3' miRNA: 3'- -CGCGu----GU-CGUC-GUGGCGGaAUAGC-CC- -5' |
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| 32863 | 5' | -56 | NC_007383.1 | + | 85799 | 0.69 | 0.724494 |
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Target: 5'- uGCGCACuGgAGCACCGCCcacGUCc-- -3' miRNA: 3'- -CGCGUGuCgUCGUGGCGGaa-UAGccc -5' |
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| 32863 | 5' | -56 | NC_007383.1 | + | 48508 | 0.7 | 0.714519 |
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Target: 5'- aGCGCACucuuuGCAGCACaucaucaGUCgaggAUUGGGu -3' miRNA: 3'- -CGCGUGu----CGUCGUGg------CGGaa--UAGCCC- -5' |
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| 32863 | 5' | -56 | NC_007383.1 | + | 26532 | 1.12 | 0.0017 |
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Target: 5'- aGCGCACAGCAGCACCGCCUUAUCGGGc -3' miRNA: 3'- -CGCGUGUCGUCGUGGCGGAAUAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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