Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32864 | 5' | -50.8 | NC_007383.1 | + | 51815 | 0.66 | 0.990091 |
Target: 5'- ---aAGgAGCGGCCACAGaUAgGUUUu -3' miRNA: 3'- guugUCgUCGCCGGUGUUaAUgCAGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 133329 | 0.66 | 0.987132 |
Target: 5'- cCAACGGUA-CuGCCGCAggAUUGgGUCCu -3' miRNA: 3'- -GUUGUCGUcGcCGGUGU--UAAUgCAGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 80939 | 0.67 | 0.983534 |
Target: 5'- ---aAGCGGCGGCCG-----ACGUCg -3' miRNA: 3'- guugUCGUCGCCGGUguuaaUGCAGg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 105193 | 0.67 | 0.983534 |
Target: 5'- aCGuCGGCAGCGGCaaaaaaCGCGAgaACGUgCu -3' miRNA: 3'- -GUuGUCGUCGCCG------GUGUUaaUGCAgG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 1300 | 0.67 | 0.983136 |
Target: 5'- gCGGCGGUGGUGGCgGCGGUggcaucgaaguCGUCg -3' miRNA: 3'- -GUUGUCGUCGCCGgUGUUAau---------GCAGg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 64028 | 0.67 | 0.981472 |
Target: 5'- gCAACAGCAuGUGGUCuCAGUacAUGUUCa -3' miRNA: 3'- -GUUGUCGU-CGCCGGuGUUAa-UGCAGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 130434 | 0.68 | 0.964517 |
Target: 5'- gCAACGGCAcCGGCCACGAUaaaccuuUAgGUa- -3' miRNA: 3'- -GUUGUCGUcGCCGGUGUUA-------AUgCAgg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 81941 | 0.68 | 0.961313 |
Target: 5'- cUAGCAcCAGCGGUagaACAAUUACGacgaCCg -3' miRNA: 3'- -GUUGUcGUCGCCGg--UGUUAAUGCa---GG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 54538 | 0.68 | 0.961313 |
Target: 5'- aUAGCAGUAGCGGUCG--AUgcCGUCg -3' miRNA: 3'- -GUUGUCGUCGCCGGUguUAauGCAGg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 6401 | 0.69 | 0.944662 |
Target: 5'- gAAUAGCAGU-GCCAUAAUaggcaugaaACGUCCg -3' miRNA: 3'- gUUGUCGUCGcCGGUGUUAa--------UGCAGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 24822 | 0.69 | 0.944662 |
Target: 5'- aCAACAGCAGCaGCaacaACAGagaaaACGUCUc -3' miRNA: 3'- -GUUGUCGUCGcCGg---UGUUaa---UGCAGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 35402 | 0.69 | 0.934805 |
Target: 5'- gCGACAGCGGUGGCaGCGAUU-CGa-- -3' miRNA: 3'- -GUUGUCGUCGCCGgUGUUAAuGCagg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 80641 | 0.7 | 0.929485 |
Target: 5'- gCGACuGCcuccugaggaagAGCGucuGCCGCGAggACGUCCg -3' miRNA: 3'- -GUUGuCG------------UCGC---CGGUGUUaaUGCAGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 23725 | 0.7 | 0.929485 |
Target: 5'- uCGGCGGCggaGGUGGCgGCGAUgAUGUCg -3' miRNA: 3'- -GUUGUCG---UCGCCGgUGUUAaUGCAGg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 32814 | 0.7 | 0.918057 |
Target: 5'- ---gGGCGGCGGCgACAaaGUUGuCGUCg -3' miRNA: 3'- guugUCGUCGCCGgUGU--UAAU-GCAGg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 18279 | 0.71 | 0.89896 |
Target: 5'- gCAACAGCAGCcGCCGCcgcgAAUUcacguacaaACGUCg -3' miRNA: 3'- -GUUGUCGUCGcCGGUG----UUAA---------UGCAGg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 80871 | 0.71 | 0.892082 |
Target: 5'- gCGGCGGCGGUGGUgGCAGgcGCGgUUCu -3' miRNA: 3'- -GUUGUCGUCGCCGgUGUUaaUGC-AGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 18433 | 0.71 | 0.884953 |
Target: 5'- -cGCGGCGGCGGCUGCuGUUGCu--- -3' miRNA: 3'- guUGUCGUCGCCGGUGuUAAUGcagg -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 36957 | 0.74 | 0.732166 |
Target: 5'- aCGAUgaaGGCGGCGGCgACAGUgGCGuaUCCg -3' miRNA: 3'- -GUUG---UCGUCGCCGgUGUUAaUGC--AGG- -5' |
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32864 | 5' | -50.8 | NC_007383.1 | + | 41011 | 0.77 | 0.562342 |
Target: 5'- gCAAUAaCGGCGGCUACGAUgauagcgccauUACGUCCu -3' miRNA: 3'- -GUUGUcGUCGCCGGUGUUA-----------AUGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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