Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32867 | 5' | -50 | NC_007383.1 | + | 1241 | 0.66 | 0.993709 |
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Target: 5'- gGGCGgagGUGGUGGCGGAGgCGGuaucaaGGACAUc -3' miRNA: 3'- -CUGU---UACCGUCGCUUUgGCU------UCUGUA- -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 117740 | 0.66 | 0.990306 |
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Target: 5'- gGAUuuUGGCAauCGAuuuuGCCGAAGGCAUc -3' miRNA: 3'- -CUGuuACCGUc-GCUu---UGGCUUCUGUA- -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 23720 | 0.67 | 0.985604 |
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Target: 5'- cGGCAucGGCGGCGGAGgUGgcGGCGa -3' miRNA: 3'- -CUGUuaCCGUCGCUUUgGCuuCUGUa -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 1301 | 0.67 | 0.979329 |
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Target: 5'- cGGCGgugGUGGCGGCGGuggcAUCGAAGuCGUn -3' miRNA: 3'- -CUGU---UACCGUCGCUu---UGGCUUCuGUA- -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 27050 | 0.71 | 0.910276 |
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Target: 5'- --aAAUGGCAGCGAAGaCGAuGACGa -3' miRNA: 3'- cugUUACCGUCGCUUUgGCUuCUGUa -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 1334 | 0.72 | 0.866976 |
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Target: 5'- cGACAuUGGCGGCGGAGgUGguGACGc -3' miRNA: 3'- -CUGUuACCGUCGCUUUgGCuuCUGUa -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 1307 | 0.74 | 0.776551 |
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Target: 5'- cGACAAUuGCGGCGgcGCCGAuAGACGc -3' miRNA: 3'- -CUGUUAcCGUCGCuuUGGCU-UCUGUa -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 35397 | 0.77 | 0.584256 |
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Target: 5'- aGCGGUGGCAGCGAuucgaguAUgGAAGACAa -3' miRNA: 3'- cUGUUACCGUCGCUu------UGgCUUCUGUa -5' |
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| 32867 | 5' | -50 | NC_007383.1 | + | 67252 | 1.06 | 0.01244 |
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Target: 5'- cGACAAUGGCAGCGAAACCGAAGACAUc -3' miRNA: 3'- -CUGUUACCGUCGCUUUGGCUUCUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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