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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32869 | 5' | -49.9 | NC_007383.1 | + | 84538 | 1.1 | 0.008617 |
Target: 5'- uUCCUCGAGCUCAGAAUACGAUGACGAu -3' miRNA: 3'- -AGGAGCUCGAGUCUUAUGCUACUGCU- -5' |
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32869 | 5' | -49.9 | NC_007383.1 | + | 3732 | 0.73 | 0.848591 |
Target: 5'- cUCCUCGGGCUCAGcGU-CGGgcucagcGACGGg -3' miRNA: 3'- -AGGAGCUCGAGUCuUAuGCUa------CUGCU- -5' |
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32869 | 5' | -49.9 | NC_007383.1 | + | 3805 | 0.66 | 0.996674 |
Target: 5'- gCgUCGAGCUCAGcGU-CGAgcucagcGACGGg -3' miRNA: 3'- aGgAGCUCGAGUCuUAuGCUa------CUGCU- -5' |
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32869 | 5' | -49.9 | NC_007383.1 | + | 95103 | 0.67 | 0.990478 |
Target: 5'- aUCC-CGAGCUgu--AUGCGGUGGCGu -3' miRNA: 3'- -AGGaGCUCGAgucuUAUGCUACUGCu -5' |
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32869 | 5' | -49.9 | NC_007383.1 | + | 63144 | 0.67 | 0.990478 |
Target: 5'- aCgUCGGGaUCAauGAAaGCGAUGACGAu -3' miRNA: 3'- aGgAGCUCgAGU--CUUaUGCUACUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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