miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33005 5' -50.8 NC_007456.1 + 16741 0.66 0.866699
Target:  5'- gCUCCAUGU--GCCAguagucugccUCAGCAguCGUa -3'
miRNA:   3'- aGAGGUACAucCGGU----------AGUUGUguGCG- -5'
33005 5' -50.8 NC_007456.1 + 7070 0.66 0.849135
Target:  5'- cCUCCAUGUAGGUaAUCuGGCAa--GCc -3'
miRNA:   3'- aGAGGUACAUCCGgUAG-UUGUgugCG- -5'
33005 5' -50.8 NC_007456.1 + 17789 0.66 0.839965
Target:  5'- --cCCAUGUugacgccagAGGCUcgCAugGCGCGg -3'
miRNA:   3'- agaGGUACA---------UCCGGuaGUugUGUGCg -5'
33005 5' -50.8 NC_007456.1 + 16200 0.66 0.837166
Target:  5'- cCUCCGUGUugguucaggaacuuGGuGCCAgCAGCGguaaucuccCACGCa -3'
miRNA:   3'- aGAGGUACA--------------UC-CGGUaGUUGU---------GUGCG- -5'
33005 5' -50.8 NC_007456.1 + 19277 0.68 0.76956
Target:  5'- cCUCCAaggugGUGGGCCucGUUAACccgaacgguauCACGCa -3'
miRNA:   3'- aGAGGUa----CAUCCGG--UAGUUGu----------GUGCG- -5'
33005 5' -50.8 NC_007456.1 + 29337 0.69 0.66829
Target:  5'- aCUCUAUGgucggcguGGCUAUCcGCGCugGUc -3'
miRNA:   3'- aGAGGUACau------CCGGUAGuUGUGugCG- -5'
33005 5' -50.8 NC_007456.1 + 18818 1.13 0.00097
Target:  5'- gUCUCCAUGUAGGCCAUCAACACACGCu -3'
miRNA:   3'- -AGAGGUACAUCCGGUAGUUGUGUGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.