Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33005 | 5' | -50.8 | NC_007456.1 | + | 16741 | 0.66 | 0.866699 |
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Target: 5'- gCUCCAUGU--GCCAguagucugccUCAGCAguCGUa -3' miRNA: 3'- aGAGGUACAucCGGU----------AGUUGUguGCG- -5' |
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| 33005 | 5' | -50.8 | NC_007456.1 | + | 7070 | 0.66 | 0.849135 |
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Target: 5'- cCUCCAUGUAGGUaAUCuGGCAa--GCc -3' miRNA: 3'- aGAGGUACAUCCGgUAG-UUGUgugCG- -5' |
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| 33005 | 5' | -50.8 | NC_007456.1 | + | 17789 | 0.66 | 0.839965 |
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Target: 5'- --cCCAUGUugacgccagAGGCUcgCAugGCGCGg -3' miRNA: 3'- agaGGUACA---------UCCGGuaGUugUGUGCg -5' |
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| 33005 | 5' | -50.8 | NC_007456.1 | + | 16200 | 0.66 | 0.837166 |
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Target: 5'- cCUCCGUGUugguucaggaacuuGGuGCCAgCAGCGguaaucuccCACGCa -3' miRNA: 3'- aGAGGUACA--------------UC-CGGUaGUUGU---------GUGCG- -5' |
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| 33005 | 5' | -50.8 | NC_007456.1 | + | 19277 | 0.68 | 0.76956 |
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Target: 5'- cCUCCAaggugGUGGGCCucGUUAACccgaacgguauCACGCa -3' miRNA: 3'- aGAGGUa----CAUCCGG--UAGUUGu----------GUGCG- -5' |
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| 33005 | 5' | -50.8 | NC_007456.1 | + | 29337 | 0.69 | 0.66829 |
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Target: 5'- aCUCUAUGgucggcguGGCUAUCcGCGCugGUc -3' miRNA: 3'- aGAGGUACau------CCGGUAGuUGUGugCG- -5' |
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| 33005 | 5' | -50.8 | NC_007456.1 | + | 18818 | 1.13 | 0.00097 |
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Target: 5'- gUCUCCAUGUAGGCCAUCAACACACGCu -3' miRNA: 3'- -AGAGGUACAUCCGGUAGUUGUGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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