Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33007 | 3' | -43.6 | NC_007456.1 | + | 18492 | 0.66 | 0.997241 |
Target: 5'- uUCGCgccuucAGCAGcGAACCCUUCAcguucAGCc -3' miRNA: 3'- -AGUGaaau--UCGUU-UUUGGGGAGU-----UCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 37365 | 0.66 | 0.997241 |
Target: 5'- cUCGCUgucccugcUUAGGUuuGAGuuCCUUAAGCu -3' miRNA: 3'- -AGUGA--------AAUUCGuuUUUggGGAGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 20026 | 0.67 | 0.995143 |
Target: 5'- aUAgUUUAGGCuuGAGCCauguCCUCAccAGCu -3' miRNA: 3'- aGUgAAAUUCGuuUUUGG----GGAGU--UCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 24282 | 0.67 | 0.994207 |
Target: 5'- gUCACcuaGGGCAAacucuGAGCCUacaUCAAGCu -3' miRNA: 3'- -AGUGaaaUUCGUU-----UUUGGGg--AGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 7438 | 0.68 | 0.993132 |
Target: 5'- aCGCUUcuGGCGA--GCCUCUUcAGCu -3' miRNA: 3'- aGUGAAauUCGUUuuUGGGGAGuUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 17359 | 0.68 | 0.991902 |
Target: 5'- aCGCUgagaagauggGAGCuauuaguCUCCUCAAGCa -3' miRNA: 3'- aGUGAaa--------UUCGuuuuu--GGGGAGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 36955 | 0.68 | 0.991902 |
Target: 5'- aCGCaUUGAGUAAAGACUuaguggcgCgUCAGGCg -3' miRNA: 3'- aGUGaAAUUCGUUUUUGG--------GgAGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 3031 | 0.68 | 0.990504 |
Target: 5'- aCACUUgAGGaCGAAAuCCgCUUCGGGCg -3' miRNA: 3'- aGUGAAaUUC-GUUUUuGG-GGAGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 4382 | 0.69 | 0.982936 |
Target: 5'- -gACUUUAAGCuc---CUCCUCAAuGCg -3' miRNA: 3'- agUGAAAUUCGuuuuuGGGGAGUU-CG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 39474 | 0.7 | 0.971517 |
Target: 5'- cCACUgUAGGuCAAGGACCgUaggUCAGGCg -3' miRNA: 3'- aGUGAaAUUC-GUUUUUGGgG---AGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 35561 | 0.72 | 0.939922 |
Target: 5'- aUCACgaauuugcUGAGCAAuAACCCCgaagugaauaCGAGCa -3' miRNA: 3'- -AGUGaa------AUUCGUUuUUGGGGa---------GUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 10785 | 0.75 | 0.83737 |
Target: 5'- gUCACUggAAGCuggcauGAAGGCUCgUCAGGCu -3' miRNA: 3'- -AGUGAaaUUCG------UUUUUGGGgAGUUCG- -5' |
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33007 | 3' | -43.6 | NC_007456.1 | + | 22380 | 1.16 | 0.003985 |
Target: 5'- cUCACUUUAAGCAAAAACCCCUCAAGCa -3' miRNA: 3'- -AGUGAAAUUCGUUUUUGGGGAGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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