Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33009 | 3' | -46.7 | NC_007456.1 | + | 38323 | 0.66 | 0.975093 |
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Target: 5'- cUGUGACgUUcuGGaGCCUAUcauggGGUCUCa -3' miRNA: 3'- aAUAUUGgAAuuCC-CGGAUA-----UCAGGG- -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 17721 | 0.66 | 0.975093 |
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Target: 5'- --uUGACCUUAAGGucuuugauaCCUuUGGUCUCa -3' miRNA: 3'- aauAUUGGAAUUCCc--------GGAuAUCAGGG- -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 16037 | 0.66 | 0.964703 |
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Target: 5'- ------aCUUAAGGGCCgcUAGgaCCCg -3' miRNA: 3'- aauauugGAAUUCCCGGauAUCa-GGG- -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 25268 | 0.67 | 0.951599 |
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Target: 5'- --cUAACUUauugAAGGGCCUAUAGauuuaCCu -3' miRNA: 3'- aauAUUGGAa---UUCCCGGAUAUCag---GG- -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 38229 | 0.68 | 0.923224 |
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Target: 5'- gUAUGuACCUUGAGcuauucaaGCCUGUAGcggCCCg -3' miRNA: 3'- aAUAU-UGGAAUUCc-------CGGAUAUCa--GGG- -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 124 | 0.7 | 0.838959 |
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Target: 5'- ---cAACCUUAugauagacugggguAGGGCCUuUGGUCUg -3' miRNA: 3'- aauaUUGGAAU--------------UCCCGGAuAUCAGGg -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 39611 | 0.7 | 0.838959 |
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Target: 5'- ---cAACCUUAugauagacugggguAGGGCCUuUGGUCUg -3' miRNA: 3'- aauaUUGGAAU--------------UCCCGGAuAUCAGGg -5' |
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| 33009 | 3' | -46.7 | NC_007456.1 | + | 25378 | 1.11 | 0.003445 |
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Target: 5'- cUUAUAACCUUAAGGGCCUAUAGUCCCu -3' miRNA: 3'- -AAUAUUGGAAUUCCCGGAUAUCAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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