Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33010 | 3' | -52.2 | NC_007456.1 | + | 30328 | 0.67 | 0.721525 |
Target: 5'- gCUGU--UACUGAGGCacucaAGGACCCUu- -3' miRNA: 3'- gGGCAuuAUGACUCCG-----UCUUGGGGuc -5' |
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33010 | 3' | -52.2 | NC_007456.1 | + | 18313 | 0.68 | 0.699141 |
Target: 5'- aCUgGUGcUGCUGGGGUuuGAGCCUCAa -3' miRNA: 3'- -GGgCAUuAUGACUCCGu-CUUGGGGUc -5' |
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33010 | 3' | -52.2 | NC_007456.1 | + | 29460 | 0.68 | 0.665067 |
Target: 5'- uUCGUAAcGCUGGGGUucuGCCUCAGu -3' miRNA: 3'- gGGCAUUaUGACUCCGucuUGGGGUC- -5' |
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33010 | 3' | -52.2 | NC_007456.1 | + | 31585 | 0.69 | 0.619218 |
Target: 5'- -gUGUAAUACUu-GGCAGu-CCCCAGa -3' miRNA: 3'- ggGCAUUAUGAcuCCGUCuuGGGGUC- -5' |
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33010 | 3' | -52.2 | NC_007456.1 | + | 29534 | 1.1 | 0.001145 |
Target: 5'- cCCCGUAAUACUGAGGCAGAACCCCAGc -3' miRNA: 3'- -GGGCAUUAUGACUCCGUCUUGGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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