miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33012 3' -52.5 NC_007456.1 + 29016 0.67 0.743523
Target:  5'- gGGuUCGAGAgGUcuagacucuggGUAGCUGGgauaccagucgCAGCCAc -3'
miRNA:   3'- -CC-AGCUCUgCG-----------UAUCGACCa----------GUUGGU- -5'
33012 3' -52.5 NC_007456.1 + 18030 0.67 0.743523
Target:  5'- aGGUCaAGAC-CGggAGUUGGUCGguGCCAa -3'
miRNA:   3'- -CCAGcUCUGcGUa-UCGACCAGU--UGGU- -5'
33012 3' -52.5 NC_007456.1 + 29343 0.67 0.732578
Target:  5'- uGGUCGGcGugGCuauccgcGCUGGUCAgACUg -3'
miRNA:   3'- -CCAGCU-CugCGuau----CGACCAGU-UGGu -5'
33012 3' -52.5 NC_007456.1 + 2833 0.67 0.699141
Target:  5'- aGGUCcAGACGgGaAGCU-GUCAACCu -3'
miRNA:   3'- -CCAGcUCUGCgUaUCGAcCAGUUGGu -5'
33012 3' -52.5 NC_007456.1 + 1754 0.71 0.506789
Target:  5'- ---gGAGACGCAUAGUcucaucaugGGUUAACCGc -3'
miRNA:   3'- ccagCUCUGCGUAUCGa--------CCAGUUGGU- -5'
33012 3' -52.5 NC_007456.1 + 30755 1.11 0.000892
Target:  5'- aGGUCGAGACGCAUAGCUGGUCAACCAu -3'
miRNA:   3'- -CCAGCUCUGCGUAUCGACCAGUUGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.