miRNA: 3'- -CUACCGGAG--GACGUUGCGG-----AGACCUG- -5'
miRNA: 3'- -CUACCGGAGGACGUUGCGGAGACCUG- -5'
Results 1 - 2 of 2 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33012 | 5' | -57.9 | NC_007456.1 | + | 12168 | 0.75 | 0.109263 |
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Target: 5'- gGAUGGUCUUgaCUGCGACGUCauacuUCUGGACc -3' miRNA: 3'- -CUACCGGAG--GACGUUGCGG-----AGACCUG- -5' |
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| 33012 | 5' | -57.9 | NC_007456.1 | + | 30721 | 1.08 | 0.000306 |
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Target: 5'- uGAUGGCCUCCUGCAACGCCUCUGGACu -3' miRNA: 3'- -CUACCGGAGGACGUUGCGGAGACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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