miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33013 3' -52 NC_007456.1 + 12917 0.68 0.577967
Target:  5'- -aAGGCCaCUCgcugugaaugaccUUGAGUCUAGACCu- -3'
miRNA:   3'- uaUCCGG-GAGa------------AAUUCAGGUCUGGuu -5'
33013 3' -52 NC_007456.1 + 1657 0.73 0.349152
Target:  5'- -cAGcGCCCggCUUauAGUCCAGACCGGg -3'
miRNA:   3'- uaUC-CGGGa-GAAauUCAGGUCUGGUU- -5'
33013 3' -52 NC_007456.1 + 30 1.05 0.002017
Target:  5'- cAUAGGCCCUCUUUAAGUCCAGACCAAa -3'
miRNA:   3'- -UAUCCGGGAGAAAUUCAGGUCUGGUU- -5'
33013 3' -52 NC_007456.1 + 39517 1.05 0.002017
Target:  5'- cAUAGGCCCUCUUUAAGUCCAGACCAAa -3'
miRNA:   3'- -UAUCCGGGAGAAAUUCAGGUCUGGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.