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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33016 | 3' | -51.8 | NC_007456.1 | + | 37603 | 0.66 | 0.824717 |
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Target: 5'- uGAGGAGuACCCagauagaugauuguGCCUCcGGGcUUCAGg -3' miRNA: 3'- gCUCUUCuUGGG--------------UGGAG-UCC-AAGUCg -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 27426 | 0.67 | 0.777562 |
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Target: 5'- gGuGAAGAACUcaCACCUCAGcgUCAGa -3' miRNA: 3'- gCuCUUCUUGG--GUGGAGUCcaAGUCg -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 23541 | 0.67 | 0.760614 |
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Target: 5'- aGAGcugcaccaauggcuuGAGcgUCCACCUUAGGUgggucauucUCGGCa -3' miRNA: 3'- gCUC---------------UUCuuGGGUGGAGUCCA---------AGUCG- -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 19534 | 0.67 | 0.756318 |
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Target: 5'- gGAGAacuGGAGgCgACCUUAGGUggcguguacUCAGUa -3' miRNA: 3'- gCUCU---UCUUgGgUGGAGUCCA---------AGUCG- -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 20895 | 0.69 | 0.632338 |
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Target: 5'- --uGAGGAGCCUuaggugcgACCaCAGGUUUAGCc -3' miRNA: 3'- gcuCUUCUUGGG--------UGGaGUCCAAGUCG- -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 4895 | 0.69 | 0.620824 |
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Target: 5'- --uGAAGGACCCuaagaACaUCAGGUUCAGa -3' miRNA: 3'- gcuCUUCUUGGG-----UGgAGUCCAAGUCg -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 23454 | 0.71 | 0.518889 |
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Target: 5'- cCGAGAAuGACCCACCUaAGGUggacgcUCAaGCc -3' miRNA: 3'- -GCUCUUcUUGGGUGGAgUCCA------AGU-CG- -5' |
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| 33016 | 3' | -51.8 | NC_007456.1 | + | 8764 | 1.14 | 0.000702 |
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Target: 5'- aCGAGAAGAACCCACCUCAGGUUCAGCg -3' miRNA: 3'- -GCUCUUCUUGGGUGGAGUCCAAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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