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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33017 | 5' | -54.2 | NC_007456.1 | + | 19667 | 0.66 | 0.682211 |
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Target: 5'- -aGCCUACUGuCUCcacUGGuCUgGAAGCg -3' miRNA: 3'- caCGGAUGGC-GAG---ACCuGAaCUUCGg -5' |
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| 33017 | 5' | -54.2 | NC_007456.1 | + | 29712 | 0.66 | 0.682211 |
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Target: 5'- -aGUCUGgUGCUCUGG-C-UGggGCa -3' miRNA: 3'- caCGGAUgGCGAGACCuGaACuuCGg -5' |
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| 33017 | 5' | -54.2 | NC_007456.1 | + | 10741 | 0.66 | 0.659448 |
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Target: 5'- gGUGCggcugaugCUGCUGCUCUGG-CUgacAAGCUg -3' miRNA: 3'- -CACG--------GAUGGCGAGACCuGAac-UUCGG- -5' |
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| 33017 | 5' | -54.2 | NC_007456.1 | + | 1732 | 0.67 | 0.579503 |
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Target: 5'- -gGUUaACCGCccggUCUGGACUauAAGCCg -3' miRNA: 3'- caCGGaUGGCG----AGACCUGAacUUCGG- -5' |
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| 33017 | 5' | -54.2 | NC_007456.1 | + | 22170 | 0.74 | 0.238937 |
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Target: 5'- -cGCCUGCUGCuUCUGGACgcaGAccaccguGGCCc -3' miRNA: 3'- caCGGAUGGCG-AGACCUGaa-CU-------UCGG- -5' |
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| 33017 | 5' | -54.2 | NC_007456.1 | + | 20391 | 0.78 | 0.136687 |
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Target: 5'- aGUGUC-ACCGCUCUGGAUgaaaacGAAGCUg -3' miRNA: 3'- -CACGGaUGGCGAGACCUGaa----CUUCGG- -5' |
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| 33017 | 5' | -54.2 | NC_007456.1 | + | 9286 | 1.12 | 0.000423 |
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Target: 5'- gGUGCCUACCGCUCUGGACUUGAAGCCa -3' miRNA: 3'- -CACGGAUGGCGAGACCUGAACUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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