miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33017 5' -54.2 NC_007456.1 + 19667 0.66 0.682211
Target:  5'- -aGCCUACUGuCUCcacUGGuCUgGAAGCg -3'
miRNA:   3'- caCGGAUGGC-GAG---ACCuGAaCUUCGg -5'
33017 5' -54.2 NC_007456.1 + 29712 0.66 0.682211
Target:  5'- -aGUCUGgUGCUCUGG-C-UGggGCa -3'
miRNA:   3'- caCGGAUgGCGAGACCuGaACuuCGg -5'
33017 5' -54.2 NC_007456.1 + 10741 0.66 0.659448
Target:  5'- gGUGCggcugaugCUGCUGCUCUGG-CUgacAAGCUg -3'
miRNA:   3'- -CACG--------GAUGGCGAGACCuGAac-UUCGG- -5'
33017 5' -54.2 NC_007456.1 + 1732 0.67 0.579503
Target:  5'- -gGUUaACCGCccggUCUGGACUauAAGCCg -3'
miRNA:   3'- caCGGaUGGCG----AGACCUGAacUUCGG- -5'
33017 5' -54.2 NC_007456.1 + 22170 0.74 0.238937
Target:  5'- -cGCCUGCUGCuUCUGGACgcaGAccaccguGGCCc -3'
miRNA:   3'- caCGGAUGGCG-AGACCUGaa-CU-------UCGG- -5'
33017 5' -54.2 NC_007456.1 + 20391 0.78 0.136687
Target:  5'- aGUGUC-ACCGCUCUGGAUgaaaacGAAGCUg -3'
miRNA:   3'- -CACGGaUGGCGAGACCUGaa----CUUCGG- -5'
33017 5' -54.2 NC_007456.1 + 9286 1.12 0.000423
Target:  5'- gGUGCCUACCGCUCUGGACUUGAAGCCa -3'
miRNA:   3'- -CACGGAUGGCGAGACCUGAACUUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.