Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33020 | 3' | -48.4 | NC_007456.1 | + | 31824 | 0.66 | 0.945589 |
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Target: 5'- --aUGGACCUgUGGCGUaugggugacACCAUCGCUg -3' miRNA: 3'- acaAUUUGGA-GUCGCA---------UGGUAGUGGu -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 13371 | 0.66 | 0.934681 |
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Target: 5'- ---cAAGCCUCaagagacuGGCGUcCCAUCAUCu -3' miRNA: 3'- acaaUUUGGAG--------UCGCAuGGUAGUGGu -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 32287 | 0.67 | 0.894207 |
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Target: 5'- ---gAAGCCUgAaCGUGCCAUCACg- -3' miRNA: 3'- acaaUUUGGAgUcGCAUGGUAGUGgu -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 682 | 0.67 | 0.88635 |
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Target: 5'- --gUAGACCauuUCAGUGUuggUCAUCACCGu -3' miRNA: 3'- acaAUUUGG---AGUCGCAu--GGUAGUGGU- -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 1341 | 0.68 | 0.878189 |
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Target: 5'- gGUcUAuACCaUCAGCa-GCCAUCACCGu -3' miRNA: 3'- aCA-AUuUGG-AGUCGcaUGGUAGUGGU- -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 16541 | 0.7 | 0.781828 |
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Target: 5'- cUGUUGAACCgUUGGUcgGCCAUCACUu -3' miRNA: 3'- -ACAAUUUGG-AGUCGcaUGGUAGUGGu -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 8911 | 0.71 | 0.702826 |
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Target: 5'- -uUUAAACUUCAugguaacaGUACCGUCACCAu -3' miRNA: 3'- acAAUUUGGAGUcg------CAUGGUAGUGGU- -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 33253 | 0.72 | 0.679308 |
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Target: 5'- aGUUGAACCagcaggaacguUCuuuggaAGCGUACCAUC-CCAg -3' miRNA: 3'- aCAAUUUGG-----------AG------UCGCAUGGUAGuGGU- -5' |
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| 33020 | 3' | -48.4 | NC_007456.1 | + | 20794 | 1.1 | 0.002919 |
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Target: 5'- gUGUUAAACCUCAGCGUACCAUCACCAc -3' miRNA: 3'- -ACAAUUUGGAGUCGCAUGGUAGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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