Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33021 | 3' | -52.9 | NC_007456.1 | + | 4338 | 0.66 | 0.72721 |
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Target: 5'- uGUCUu-GGACUUUACCGGaAGGCGUc -3' miRNA: 3'- uCGGGuuUCUGAGGUGGUCaUUCGCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 26574 | 0.67 | 0.693424 |
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Target: 5'- cGCCCGAgccAGACcugagUCACCAGUugGAGCc- -3' miRNA: 3'- uCGGGUU---UCUGa----GGUGGUCA--UUCGcg -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 16113 | 0.67 | 0.682014 |
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Target: 5'- uGUCCAc-GACUcgcagccucagCCACCAGUGcAGCGUu -3' miRNA: 3'- uCGGGUuuCUGA-----------GGUGGUCAU-UCGCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 22800 | 0.68 | 0.635975 |
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Target: 5'- uAGCCCAGAGucugaACUgUACCuucGUGAGCa- -3' miRNA: 3'- -UCGGGUUUC-----UGAgGUGGu--CAUUCGcg -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 33979 | 0.68 | 0.624426 |
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Target: 5'- cAGCUUuaguGAUaCCACCAGUAAGaUGCa -3' miRNA: 3'- -UCGGGuuu-CUGaGGUGGUCAUUC-GCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 7374 | 0.68 | 0.601362 |
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Target: 5'- aAGCUgaAGAGGCU-CGCCAG-AAGCGUg -3' miRNA: 3'- -UCGGg-UUUCUGAgGUGGUCaUUCGCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 29663 | 0.69 | 0.522052 |
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Target: 5'- cAGCaCCAAucACaccagCCGCCAGuUGAGCGCc -3' miRNA: 3'- -UCG-GGUUucUGa----GGUGGUC-AUUCGCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 16001 | 0.7 | 0.467943 |
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Target: 5'- uGCCCGAAGAC-CUuCCAGUccaacuauguGCGCa -3' miRNA: 3'- uCGGGUUUCUGaGGuGGUCAuu--------CGCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 19666 | 0.71 | 0.436893 |
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Target: 5'- gAGCCUAcuGuCUCCACUGGUcuggAAGCGUu -3' miRNA: 3'- -UCGGGUuuCuGAGGUGGUCA----UUCGCG- -5' |
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| 33021 | 3' | -52.9 | NC_007456.1 | + | 29083 | 1.12 | 0.000631 |
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Target: 5'- cAGCCCAAAGACUCCACCAGUAAGCGCu -3' miRNA: 3'- -UCGGGUUUCUGAGGUGGUCAUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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