Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33021 | 5' | -54.9 | NC_007456.1 | + | 6757 | 0.67 | 0.550345 |
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Target: 5'- uCGUACaaaUCAC-GAAGaGACUCAAGGUUg -3' miRNA: 3'- uGCGUG---AGUGcCUUC-CUGAGUUCCGA- -5' |
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| 33021 | 5' | -54.9 | NC_007456.1 | + | 20748 | 0.68 | 0.495761 |
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Target: 5'- -aGUGCUCacgaguugACGGGucguuGGACUCAAGGUg -3' miRNA: 3'- ugCGUGAG--------UGCCUu----CCUGAGUUCCGa -5' |
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| 33021 | 5' | -54.9 | NC_007456.1 | + | 31802 | 0.69 | 0.432735 |
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Target: 5'- cACGCgucacugACUCACGGAugaGGGACUUAAuGCc -3' miRNA: 3'- -UGCG-------UGAGUGCCU---UCCUGAGUUcCGa -5' |
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| 33021 | 5' | -54.9 | NC_007456.1 | + | 37787 | 0.7 | 0.401592 |
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Target: 5'- aGCGUAcCUCGuCGGucggacgccaguagAAGGACUCAGGGUc -3' miRNA: 3'- -UGCGU-GAGU-GCC--------------UUCCUGAGUUCCGa -5' |
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| 33021 | 5' | -54.9 | NC_007456.1 | + | 11752 | 0.71 | 0.341201 |
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Target: 5'- cCGCAC--ACGGGAGGcaauGCUUAAGGCa -3' miRNA: 3'- uGCGUGagUGCCUUCC----UGAGUUCCGa -5' |
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| 33021 | 5' | -54.9 | NC_007456.1 | + | 29120 | 1.08 | 0.000757 |
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Target: 5'- gACGCACUCACGGAAGGACUCAAGGCUa -3' miRNA: 3'- -UGCGUGAGUGCCUUCCUGAGUUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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