miRNA: 3'- acGUCGGUUACGAAggCGuu---GACUac -5'
miRNA: 3'- -ACGUcGGUU-------------ACGaAGGCGUUGACUac -5'
miRNA: 3'- aCGUCGGUU---ACGAAGGcGUUGA--CUac -5'
miRNA: 3'- -ACGUCGGUUACGAAGGCGUUGACUAC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33024 | 5' | -51.6 | NC_007458.1 | + | 14578 | 0.66 | 0.756607 |
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Target: 5'- -uCAGCUAGUGCUUuuGCugcuuCUGAc- -3' miRNA: 3'- acGUCGGUUACGAAggCGuu---GACUac -5' |
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| 33024 | 5' | -51.6 | NC_007458.1 | + | 9149 | 0.69 | 0.592262 |
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Target: 5'- cUGCA-CCAGauaaaauauuggcUGCaUCCGCAACUGAa- -3' miRNA: 3'- -ACGUcGGUU-------------ACGaAGGCGUUGACUac -5' |
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| 33024 | 5' | -51.6 | NC_007458.1 | + | 9024 | 0.72 | 0.395848 |
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Target: 5'- aGCAGCCGAaauUGCUUCCaCAGCUcuGAa- -3' miRNA: 3'- aCGUCGGUU---ACGAAGGcGUUGA--CUac -5' |
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| 33024 | 5' | -51.6 | NC_007458.1 | + | 9107 | 1.09 | 0.001178 |
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Target: 5'- gUGCAGCCAAUGCUUCCGCAACUGAUGu -3' miRNA: 3'- -ACGUCGGUUACGAAGGCGUUGACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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