Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33033 | 3' | -63.7 | NC_007497.1 | + | 38115 | 0.69 | 0.177648 |
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Target: 5'- gAAGGAUuCGCCG-GGuaucgcaaCCGCCGGUGUc -3' miRNA: 3'- gUUCCUAcGCGGCgCC--------GGCGGCCGCG- -5' |
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| 33033 | 3' | -63.7 | NC_007497.1 | + | 38226 | 0.79 | 0.027342 |
|
Target: 5'- -cAGGc-GCGCCaCGGUCGCCGGCGCg -3' miRNA: 3'- guUCCuaCGCGGcGCCGGCGGCCGCG- -5' |
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| 33033 | 3' | -63.7 | NC_007497.1 | + | 40115 | 0.68 | 0.197005 |
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Target: 5'- -cGGGAUcGCGCaGCGGCuucaCGCCGuucGCGCc -3' miRNA: 3'- guUCCUA-CGCGgCGCCG----GCGGC---CGCG- -5' |
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| 33033 | 3' | -63.7 | NC_007497.1 | + | 43020 | 0.66 | 0.266356 |
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Target: 5'- aCAAGGccgGCGUCGaccCGGCCGguaUCGGgGCu -3' miRNA: 3'- -GUUCCua-CGCGGC---GCCGGC---GGCCgCG- -5' |
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| 33033 | 3' | -63.7 | NC_007497.1 | + | 43158 | 0.68 | 0.182323 |
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Target: 5'- --uGGA-GCGCCGCaaagaGauugCGCCGGCGCu -3' miRNA: 3'- guuCCUaCGCGGCGc----Cg---GCGGCCGCG- -5' |
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| 33033 | 3' | -63.7 | NC_007497.1 | + | 44639 | 0.68 | 0.207359 |
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Target: 5'- aCAAGGc-GCGaCC-CGGCCGCCGaUGCu -3' miRNA: 3'- -GUUCCuaCGC-GGcGCCGGCGGCcGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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