Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33033 | 5' | -56.4 | NC_007497.1 | + | 10845 | 0.66 | 0.568566 |
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Target: 5'- aGGGCGGcAGaGGCAugcuuacGGugCGCcuacAUGUCg -3' miRNA: 3'- -CCCGUCuUCaCCGU-------CCugGCG----UACAG- -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 18341 | 0.67 | 0.536883 |
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Target: 5'- aGGGCGGAAGauauUGGgGGGGCaaaGCAa--- -3' miRNA: 3'- -CCCGUCUUC----ACCgUCCUGg--CGUacag -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 32585 | 0.67 | 0.526096 |
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Target: 5'- cGGCGGAc-UGGCGGG-CgGCcUGUCg -3' miRNA: 3'- cCCGUCUucACCGUCCuGgCGuACAG- -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 43238 | 0.67 | 0.504777 |
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Target: 5'- gGGGCuc-AGUGcGguGGGCCGCGUa-- -3' miRNA: 3'- -CCCGucuUCAC-CguCCUGGCGUAcag -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 8699 | 0.68 | 0.473527 |
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Target: 5'- -aGCGGAAGcgGGCGGGgcgcgucgGCCGCccgGUCg -3' miRNA: 3'- ccCGUCUUCa-CCGUCC--------UGGCGua-CAG- -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 28625 | 0.68 | 0.453242 |
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Target: 5'- aGGGUGGAGGcGGCGGcGGCgGCGguggagaGUCg -3' miRNA: 3'- -CCCGUCUUCaCCGUC-CUGgCGUa------CAG- -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 42241 | 0.68 | 0.43344 |
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Target: 5'- -aGCAGccGgugGGCAGGAUCGUccGUCa -3' miRNA: 3'- ccCGUCuuCa--CCGUCCUGGCGuaCAG- -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 17064 | 0.69 | 0.404705 |
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Target: 5'- gGGGCGGAAGgGGCGGaACCG-AUGg- -3' miRNA: 3'- -CCCGUCUUCaCCGUCcUGGCgUACag -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 23248 | 0.8 | 0.07814 |
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Target: 5'- cGGCGGAAGUGGCGGGAUgGCGg--- -3' miRNA: 3'- cCCGUCUUCACCGUCCUGgCGUacag -5' |
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| 33033 | 5' | -56.4 | NC_007497.1 | + | 2587 | 1.12 | 0.000295 |
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Target: 5'- cGGGCAGAAGUGGCAGGACCGCAUGUCg -3' miRNA: 3'- -CCCGUCUUCACCGUCCUGGCGUACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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