Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33034 | 5' | -55.3 | NC_007497.1 | + | 29767 | 0.67 | 0.612128 |
Target: 5'- -cGUCCGCGuAGucuUCGGGcgCgagCGCGa -3' miRNA: 3'- aaUAGGCGCuUCu--GGCCCuaGa--GCGC- -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 8885 | 0.67 | 0.58985 |
Target: 5'- -gAUCCGUGccguuGAGGCCGGGAcCg-GCGa -3' miRNA: 3'- aaUAGGCGC-----UUCUGGCCCUaGagCGC- -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 15307 | 0.67 | 0.58985 |
Target: 5'- -cGUCCGCGGgaugcgccacAGGCgCGGGGgcuucacgagCUCGUGg -3' miRNA: 3'- aaUAGGCGCU----------UCUG-GCCCUa---------GAGCGC- -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 5882 | 0.68 | 0.534945 |
Target: 5'- ---gCCGaCGAAGcuGCCGGGcggaCUCGCGg -3' miRNA: 3'- aauaGGC-GCUUC--UGGCCCua--GAGCGC- -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 14611 | 0.68 | 0.524165 |
Target: 5'- aUGUCaCGCucgaaAAGGCCGGGGaugcggacauUCUCGCa -3' miRNA: 3'- aAUAG-GCGc----UUCUGGCCCU----------AGAGCGc -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 32868 | 0.68 | 0.524165 |
Target: 5'- gUGUUgGCGcGAG-CCGGGcgCUCGCu -3' miRNA: 3'- aAUAGgCGC-UUCuGGCCCuaGAGCGc -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 27595 | 0.7 | 0.402006 |
Target: 5'- --uUCCGCGGcaucgccuacuggGGGCCGGGAgcugUCgUCGCa -3' miRNA: 3'- aauAGGCGCU-------------UCUGGCCCU----AG-AGCGc -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 32728 | 0.73 | 0.25602 |
Target: 5'- --uUCCGCGGGcuugucgacagcauGAUCGcGGAUCUCGCGc -3' miRNA: 3'- aauAGGCGCUU--------------CUGGC-CCUAGAGCGC- -5' |
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33034 | 5' | -55.3 | NC_007497.1 | + | 2764 | 1.06 | 0.001335 |
Target: 5'- gUUAUCCGCGAAGACCGGGAUCUCGCGc -3' miRNA: 3'- -AAUAGGCGCUUCUGGCCCUAGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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