Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33035 | 3' | -64.7 | NC_007497.1 | + | 2610 | 0.66 | 0.267681 |
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Target: 5'- cCGCGGccGCCGgcgcgcacGCCCGGGCagaagUGGCAg -3' miRNA: 3'- -GCGCCucUGGC--------CGGGCCCGga---GCUGU- -5' |
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| 33035 | 3' | -64.7 | NC_007497.1 | + | 1582 | 0.66 | 0.261281 |
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Target: 5'- -uCGGuGACCGGCgCGGaGCUcaucgcauUCGACGa -3' miRNA: 3'- gcGCCuCUGGCCGgGCC-CGG--------AGCUGU- -5' |
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| 33035 | 3' | -64.7 | NC_007497.1 | + | 2891 | 0.66 | 0.261281 |
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Target: 5'- aCGCGGugcuggcgcauGGugCGGauaUGGGCCUCGGgGa -3' miRNA: 3'- -GCGCC-----------UCugGCCgg-GCCCGGAGCUgU- -5' |
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| 33035 | 3' | -64.7 | NC_007497.1 | + | 36032 | 0.68 | 0.179625 |
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Target: 5'- aCGCGGAaGCCGcGgCCGcGGCgUUCGGCAu -3' miRNA: 3'- -GCGCCUcUGGC-CgGGC-CCG-GAGCUGU- -5' |
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| 33035 | 3' | -64.7 | NC_007497.1 | + | 36593 | 0.69 | 0.162037 |
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Target: 5'- uCGUGu--GCCGGCCCGuacGCCUCGGCGa -3' miRNA: 3'- -GCGCcucUGGCCGGGCc--CGGAGCUGU- -5' |
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| 33035 | 3' | -64.7 | NC_007497.1 | + | 3258 | 1.07 | 0.000148 |
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Target: 5'- uCGCGGAGACCGGCCCGGGCCUCGACAg -3' miRNA: 3'- -GCGCCUCUGGCCGGGCCCGGAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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