Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33036 | 3' | -58.2 | NC_007497.1 | + | 34852 | 0.66 | 0.490523 |
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Target: 5'- cGCGGC-CGcGGUCuCGcgcGCGAUCACg- -3' miRNA: 3'- cUGCCGaGC-CCAG-GCa--CGCUAGUGac -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 28767 | 0.67 | 0.47031 |
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Target: 5'- cGCGGCUCaGGGggagcCCGUGC---CGCUGa -3' miRNA: 3'- cUGCCGAG-CCCa----GGCACGcuaGUGAC- -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 27239 | 0.67 | 0.440814 |
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Target: 5'- gGACGGCgugCuggucGGUCCGgggcagGUGAUCGCUa -3' miRNA: 3'- -CUGCCGa--Gc----CCAGGCa-----CGCUAGUGAc -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 29151 | 0.67 | 0.421743 |
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Target: 5'- cGACaGC-CGaGG-CCGUGCGggCACUGu -3' miRNA: 3'- -CUGcCGaGC-CCaGGCACGCuaGUGAC- -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 26322 | 0.68 | 0.402264 |
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Target: 5'- cGCGGCUCGGcGUCCG-GCuguaccagaucucGGUgCGCUGc -3' miRNA: 3'- cUGCCGAGCC-CAGGCaCG-------------CUA-GUGAC- -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 13937 | 0.69 | 0.342439 |
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Target: 5'- cGGCGGCccaugCGGcGUCCGUGCGGccCAUg- -3' miRNA: 3'- -CUGCCGa----GCC-CAGGCACGCUa-GUGac -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 34892 | 0.69 | 0.334319 |
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Target: 5'- gGGCGaGCUCGaGcGUCCGagGCG-UCGCUGg -3' miRNA: 3'- -CUGC-CGAGC-C-CAGGCa-CGCuAGUGAC- -5' |
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| 33036 | 3' | -58.2 | NC_007497.1 | + | 5789 | 0.93 | 0.006149 |
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Target: 5'- cGACGGCUCGGuG-CCGUGCGAUCACUGa -3' miRNA: 3'- -CUGCCGAGCC-CaGGCACGCUAGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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