Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33037 | 5' | -58.7 | NC_007497.1 | + | 15678 | 0.66 | 0.458544 |
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Target: 5'- gUCGCGuGugcauuacuGGCC-CUCGGAUUGCg-- -3' miRNA: 3'- gAGCGC-Cu--------CCGGcGAGCCUAACGacu -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 8098 | 0.66 | 0.448725 |
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Target: 5'- aCUCGCGGuucGGUgGCUgGGAUgggaGCUa- -3' miRNA: 3'- -GAGCGCCu--CCGgCGAgCCUAa---CGAcu -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 10108 | 0.66 | 0.448724 |
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Target: 5'- -gCGCaGGcuGCCGCUCGGAUcGCa-- -3' miRNA: 3'- gaGCG-CCucCGGCGAGCCUAaCGacu -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 17224 | 0.67 | 0.383455 |
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Target: 5'- -cCGCGcAGGCagaCGCUCGGGccggGCUGAc -3' miRNA: 3'- gaGCGCcUCCG---GCGAGCCUaa--CGACU- -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 11621 | 0.67 | 0.383455 |
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Target: 5'- -cCGCGcGAGGCCaaggGCUgGG--UGCUGAg -3' miRNA: 3'- gaGCGC-CUCCGG----CGAgCCuaACGACU- -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 2291 | 0.68 | 0.33276 |
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Target: 5'- uCUUGCGcGGGGCCuGCUCGGGcaacGgUGAg -3' miRNA: 3'- -GAGCGC-CUCCGG-CGAGCCUaa--CgACU- -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 43597 | 0.7 | 0.273093 |
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Target: 5'- gUCGacCGGAaGUCGCUCGGcgUGCUGu -3' miRNA: 3'- gAGC--GCCUcCGGCGAGCCuaACGACu -5' |
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| 33037 | 5' | -58.7 | NC_007497.1 | + | 6246 | 1.08 | 0.000409 |
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Target: 5'- uCUCGCGGAGGCCGCUCGGAUUGCUGAu -3' miRNA: 3'- -GAGCGCCUCCGGCGAGCCUAACGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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