Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33038 | 3' | -58.8 | NC_007497.1 | + | 20383 | 0.66 | 0.497926 |
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Target: 5'- gGCACUCGgcGUauUCCUGcGcGCGAUCGa -3' miRNA: 3'- -CGUGAGCuaCAgcGGGAC-C-CGCUGGC- -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 13291 | 0.66 | 0.497926 |
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Target: 5'- aCGCUCGAUGcgcuUCGCaaagCUGGcGCGugCu -3' miRNA: 3'- cGUGAGCUAC----AGCGg---GACC-CGCugGc -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 3542 | 0.67 | 0.458034 |
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Target: 5'- uGCGCUU--UGUgGCCgUGaaGGCGGCCGc -3' miRNA: 3'- -CGUGAGcuACAgCGGgAC--CCGCUGGC- -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 32829 | 0.67 | 0.448329 |
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Target: 5'- cCGC-CGAgaaCGCCgCUGGGCGcGCCGa -3' miRNA: 3'- cGUGaGCUacaGCGG-GACCCGC-UGGC- -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 26038 | 0.67 | 0.429266 |
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Target: 5'- uGCAgUCGgcGaUUGCCgaGGGCGACa- -3' miRNA: 3'- -CGUgAGCuaC-AGCGGgaCCCGCUGgc -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 35842 | 0.68 | 0.375074 |
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Target: 5'- uGCGCgaugCGcagcacGUCGUCCUGcGCGACCGc -3' miRNA: 3'- -CGUGa---GCua----CAGCGGGACcCGCUGGC- -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 6919 | 0.68 | 0.358068 |
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Target: 5'- -uGC-CGAUcccgGUCGCCCaGGGCGACa- -3' miRNA: 3'- cgUGaGCUA----CAGCGGGaCCCGCUGgc -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 2425 | 0.69 | 0.325703 |
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Target: 5'- cGCGCUuagcagugcCGGUG-CGCCgCUGGGCGuuCGc -3' miRNA: 3'- -CGUGA---------GCUACaGCGG-GACCCGCugGC- -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 34834 | 0.79 | 0.071084 |
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Target: 5'- aCGCUCGAgcUCGCCCUGcGCGGCCGc -3' miRNA: 3'- cGUGAGCUacAGCGGGACcCGCUGGC- -5' |
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| 33038 | 3' | -58.8 | NC_007497.1 | + | 6997 | 1.11 | 0.000293 |
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Target: 5'- gGCACUCGAUGUCGCCCUGGGCGACCGg -3' miRNA: 3'- -CGUGAGCUACAGCGGGACCCGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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