Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33039 | 5' | -58.1 | NC_007497.1 | + | 32566 | 0.66 | 0.5037 |
Target: 5'- cUGUCGGauggcgucGCGGCCGccgccGGCGGCAacGCGUa- -3' miRNA: 3'- -ACAGCU--------CGUCGGU-----CUGCCGU--CGCAcu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 28623 | 0.66 | 0.483015 |
Target: 5'- gGUgGAgGCGGC--GGCGGCGGCGgugGAg -3' miRNA: 3'- aCAgCU-CGUCGguCUGCCGUCGCa--CU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 44457 | 0.66 | 0.472828 |
Target: 5'- cGUCcacaAGCGGCUgggcgaGGGCGGCaagaaggaacAGCGUGAc -3' miRNA: 3'- aCAGc---UCGUCGG------UCUGCCG----------UCGCACU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 35786 | 0.66 | 0.462749 |
Target: 5'- gGUCGAGacgauCAGCaAGGCGGUGGCGcUGu -3' miRNA: 3'- aCAGCUC-----GUCGgUCUGCCGUCGC-ACu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 23287 | 0.66 | 0.461748 |
Target: 5'- gGUCGAcacgcugcugcagGCuGCCAG-CGGCAGCa--- -3' miRNA: 3'- aCAGCU-------------CGuCGGUCuGCCGUCGcacu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 25454 | 0.66 | 0.452785 |
Target: 5'- -aUCGGcuGguGCUGGACGGCAguaGCGUGc -3' miRNA: 3'- acAGCU--CguCGGUCUGCCGU---CGCACu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 17275 | 0.66 | 0.452785 |
Target: 5'- cUGcgCGGGCGGCUgcgGGGCGGCcGCGa-- -3' miRNA: 3'- -ACa-GCUCGUCGG---UCUGCCGuCGCacu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 23581 | 0.67 | 0.423615 |
Target: 5'- gGUCGcGCuGCUGGgcGCGGCGGCGg-- -3' miRNA: 3'- aCAGCuCGuCGGUC--UGCCGUCGCacu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 34404 | 0.67 | 0.42076 |
Target: 5'- gGUCGAGCgccAGCgAGugaagcaggcgcaaGCGGCGGCGa-- -3' miRNA: 3'- aCAGCUCG---UCGgUC--------------UGCCGUCGCacu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 4098 | 0.67 | 0.404805 |
Target: 5'- --aCGcGGCGGCCgAGACGGCAcgGCGcGAa -3' miRNA: 3'- acaGC-UCGUCGG-UCUGCCGU--CGCaCU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 39436 | 0.68 | 0.368809 |
Target: 5'- --cCGAGguGCCGGACaugccggauguGGCGGCGa-- -3' miRNA: 3'- acaGCUCguCGGUCUG-----------CCGUCGCacu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 36167 | 0.7 | 0.29172 |
Target: 5'- cGUCGA-CGGCCGG-CGGCuauuucaacgcgguGCGUGAu -3' miRNA: 3'- aCAGCUcGUCGGUCuGCCGu-------------CGCACU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 26250 | 0.7 | 0.288797 |
Target: 5'- cGUCGGGCcaugguauccGGCC-GACGGUcccGGCGUGc -3' miRNA: 3'- aCAGCUCG----------UCGGuCUGCCG---UCGCACu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 40210 | 0.7 | 0.28807 |
Target: 5'- -aUCGcGGCAGCgCAGAUGGgcgcgaaCGGCGUGAa -3' miRNA: 3'- acAGC-UCGUCG-GUCUGCC-------GUCGCACU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 35346 | 0.71 | 0.235112 |
Target: 5'- cGUCGcuuGCAGCCGGGCGGCu-CGUc- -3' miRNA: 3'- aCAGCu--CGUCGGUCUGCCGucGCAcu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 30214 | 0.71 | 0.22307 |
Target: 5'- -aUCGAGCGGC--GACGaCAGCGUGAa -3' miRNA: 3'- acAGCUCGUCGguCUGCcGUCGCACU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 31813 | 0.72 | 0.211556 |
Target: 5'- -aUCGccccuGCGGCCGGAgCGGCGGCGUc- -3' miRNA: 3'- acAGCu----CGUCGGUCU-GCCGUCGCAcu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 19905 | 0.73 | 0.184993 |
Target: 5'- -aUCGAGUcaGGCCAGaccgucguGCGaGCAGCGUGGg -3' miRNA: 3'- acAGCUCG--UCGGUC--------UGC-CGUCGCACU- -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 35228 | 0.73 | 0.180047 |
Target: 5'- aGUCGAucGCAGCCuGACGgGCAGCa--- -3' miRNA: 3'- aCAGCU--CGUCGGuCUGC-CGUCGcacu -5' |
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33039 | 5' | -58.1 | NC_007497.1 | + | 42237 | 0.74 | 0.15277 |
Target: 5'- -cUUGAGCAGCCGGugGGCAGgauCGUc- -3' miRNA: 3'- acAGCUCGUCGGUCugCCGUC---GCAcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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