Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33040 | 3' | -57.3 | NC_007497.1 | + | 33012 | 0.66 | 0.549243 |
Target: 5'- --gCCUgauucAGCUCgCGCGcgUUGUCGCCCa -3' miRNA: 3'- uaaGGA-----UCGAG-GUGCa-AGCGGCGGGg -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 15091 | 0.66 | 0.549243 |
Target: 5'- --aCCUGuGCUuugcguugaaCCGCGUggcgCGCCGCuaCCCg -3' miRNA: 3'- uaaGGAU-CGA----------GGUGCAa---GCGGCG--GGG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 36617 | 0.66 | 0.527755 |
Target: 5'- -aUCg-AGCUCgGCGgccUCGCCGCagaCCg -3' miRNA: 3'- uaAGgaUCGAGgUGCa--AGCGGCGg--GG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 33994 | 0.66 | 0.517128 |
Target: 5'- -gUCCaucGCUgCCAgCGUgCGCgCGCCCCu -3' miRNA: 3'- uaAGGau-CGA-GGU-GCAaGCG-GCGGGG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 7270 | 0.67 | 0.448439 |
Target: 5'- cUUCCgAGUUCCccucgauGCcgauacucgcagcaaGUUCGCCGCCCUc -3' miRNA: 3'- uAAGGaUCGAGG-------UG---------------CAAGCGGCGGGG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 33239 | 0.67 | 0.44548 |
Target: 5'- -gUCCgacAGCUUCguGCGcUUCGCCGCCa- -3' miRNA: 3'- uaAGGa--UCGAGG--UGC-AAGCGGCGGgg -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 22925 | 0.68 | 0.397844 |
Target: 5'- --cCCgacgAGUcagggCCGCuGUcgUCGCCGCCCCg -3' miRNA: 3'- uaaGGa---UCGa----GGUG-CA--AGCGGCGGGG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 41164 | 0.7 | 0.312957 |
Target: 5'- --gCCgccGCaucgUCgGCGUUCGCCGCCCg -3' miRNA: 3'- uaaGGau-CG----AGgUGCAAGCGGCGGGg -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 36263 | 0.72 | 0.224134 |
Target: 5'- -cUCCUgAGCaucuUCCGac-UCGCCGCCCCa -3' miRNA: 3'- uaAGGA-UCG----AGGUgcaAGCGGCGGGG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 36769 | 0.73 | 0.212541 |
Target: 5'- -gUCC-GGCUCgACGccCGCCGCCUCg -3' miRNA: 3'- uaAGGaUCGAGgUGCaaGCGGCGGGG- -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 33391 | 0.74 | 0.180819 |
Target: 5'- uUUCC-GGCUCggaACGggCGCCGCCCg -3' miRNA: 3'- uAAGGaUCGAGg--UGCaaGCGGCGGGg -5' |
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33040 | 3' | -57.3 | NC_007497.1 | + | 10981 | 1.09 | 0.000429 |
Target: 5'- cAUUCCUAGCUCCACGUUCGCCGCCCCu -3' miRNA: 3'- -UAAGGAUCGAGGUGCAAGCGGCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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