Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33040 | 5' | -55.1 | NC_007497.1 | + | 23569 | 0.66 | 0.646528 |
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Target: 5'- -gGGCGCGGCGg-CGG-GCgccgGCGUc- -3' miRNA: 3'- caUCGCGUCGCaaGCCaCGa---CGCAac -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 1418 | 0.66 | 0.623899 |
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Target: 5'- cGUGGCGCAGCGUcCGauUGC-GCGg-- -3' miRNA: 3'- -CAUCGCGUCGCAaGCc-ACGaCGCaac -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 41295 | 0.66 | 0.600173 |
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Target: 5'- uUGGCGCGGCucaaUUCGacGUGCggccggaUGCGUUGg -3' miRNA: 3'- cAUCGCGUCGc---AAGC--CACG-------ACGCAAC- -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 36751 | 0.67 | 0.590039 |
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Target: 5'- cGUAcGCGCGGCuguucGUUCGcGUGCUGUa--- -3' miRNA: 3'- -CAU-CGCGUCG-----CAAGC-CACGACGcaac -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 32328 | 0.67 | 0.545453 |
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Target: 5'- uUGGCgGCAGCGgugcUGGUGCUGgCGg-- -3' miRNA: 3'- cAUCG-CGUCGCaa--GCCACGAC-GCaac -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 19052 | 0.68 | 0.523558 |
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Target: 5'- --uGCGgAGCGguaUCGGcUGCUGCGa-- -3' miRNA: 3'- cauCGCgUCGCa--AGCC-ACGACGCaac -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 29282 | 0.69 | 0.429995 |
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Target: 5'- uGUGGCGCgacAGCuGUaccaCGGUGCUGCGc-- -3' miRNA: 3'- -CAUCGCG---UCG-CAa---GCCACGACGCaac -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 14551 | 0.7 | 0.373354 |
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Target: 5'- -cGGUGCgauGGCGUUCaGGUcGCUGuCGUUGg -3' miRNA: 3'- caUCGCG---UCGCAAG-CCA-CGAC-GCAAC- -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 42417 | 0.72 | 0.290796 |
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Target: 5'- -gAGCGCuGCGaaacauggCGGUGCUGCGcUGc -3' miRNA: 3'- caUCGCGuCGCaa------GCCACGACGCaAC- -5' |
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| 33040 | 5' | -55.1 | NC_007497.1 | + | 10944 | 0.99 | 0.003963 |
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Target: 5'- gGUAGCGCAGCGUUCGGUGC-GCGUUGg -3' miRNA: 3'- -CAUCGCGUCGCAAGCCACGaCGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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