Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33041 | 3' | -52.1 | NC_007497.1 | + | 32513 | 0.68 | 0.740919 |
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Target: 5'- uGCGACcGGcgGCCACGUCACggggccgggcaCGUCGa -3' miRNA: 3'- -CGCUGcUCaaUGGUGCGGUGa----------GUAGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 32980 | 0.67 | 0.792921 |
|
Target: 5'- gGCGACGGaacucaucGCuCACGCgGCUCAUUGc -3' miRNA: 3'- -CGCUGCUcaa-----UG-GUGCGgUGAGUAGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 34186 | 0.66 | 0.858023 |
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Target: 5'- cGCG-CGAGc--UUGCGCgACUCGUCGa -3' miRNA: 3'- -CGCuGCUCaauGGUGCGgUGAGUAGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 34488 | 0.67 | 0.792921 |
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Target: 5'- cGCGAcguCGAGUuugaucgacaUGCagGCGCCGCUCgAUCa -3' miRNA: 3'- -CGCU---GCUCA----------AUGg-UGCGGUGAG-UAGc -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 35895 | 0.72 | 0.507816 |
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Target: 5'- cGUGACGAucuugagccgGUUGCUcagguuCGUCACUCGUCGg -3' miRNA: 3'- -CGCUGCU----------CAAUGGu-----GCGGUGAGUAGC- -5' |
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| 33041 | 3' | -52.1 | NC_007497.1 | + | 38004 | 0.67 | 0.81257 |
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Target: 5'- uUGGCGAGUcUGCCuuGCCcUUCGUCc -3' miRNA: 3'- cGCUGCUCA-AUGGugCGGuGAGUAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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