Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33042 | 3' | -55.6 | NC_007497.1 | + | 44717 | 0.66 | 0.670877 |
Target: 5'- cCCgcgacAGCAUCGgcggccgGGUCGCGCCUuguuaaagauaagGAUGu -3' miRNA: 3'- -GGac---UCGUAGUa------CCAGCGCGGG-------------CUAC- -5' |
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33042 | 3' | -55.6 | NC_007497.1 | + | 33132 | 0.67 | 0.550431 |
Target: 5'- uCC-GAGCGUCAgcgagaGGUCGCGCgaGAg- -3' miRNA: 3'- -GGaCUCGUAGUa-----CCAGCGCGggCUac -5' |
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33042 | 3' | -55.6 | NC_007497.1 | + | 3052 | 0.68 | 0.497098 |
Target: 5'- aUCUGAGCGUCGUGGauuUCGaGUgCGAUu -3' miRNA: 3'- -GGACUCGUAGUACC---AGCgCGgGCUAc -5' |
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33042 | 3' | -55.6 | NC_007497.1 | + | 43852 | 0.69 | 0.465219 |
Target: 5'- --cGAGCGUCguugaagGUGGUUGCGgCCGAc- -3' miRNA: 3'- ggaCUCGUAG-------UACCAGCGCgGGCUac -5' |
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33042 | 3' | -55.6 | NC_007497.1 | + | 12680 | 0.72 | 0.320991 |
Target: 5'- cCCUGAGCAUCGUGuGcaacuagUGCGCCgGGa- -3' miRNA: 3'- -GGACUCGUAGUAC-Ca------GCGCGGgCUac -5' |
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33042 | 3' | -55.6 | NC_007497.1 | + | 21173 | 0.75 | 0.190661 |
Target: 5'- --aGAGCGUCGUacggcgccuGUCGUGCCCGAUGa -3' miRNA: 3'- ggaCUCGUAGUAc--------CAGCGCGGGCUAC- -5' |
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33042 | 3' | -55.6 | NC_007497.1 | + | 12301 | 1.11 | 0.000533 |
Target: 5'- cCCUGAGCAUCAUGGUCGCGCCCGAUGg -3' miRNA: 3'- -GGACUCGUAGUACCAGCGCGGGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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